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首页> 外文期刊>The journal of physical chemistry, A. Molecules, spectroscopy, kinetics, environment, & general theory >Nucleotide Insertion in Cationic Bilayers
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Nucleotide Insertion in Cationic Bilayers

机译：阳离子双层中的核苷酸插入

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摘要

The interaction between 2'-deoxyadenosine 5'-monophosphate (DMP) and cationic liposomes made up of dioctadecyldimethylammonium bromide (DODAB) in water is described. At maximal adsorption, the molar ratio DODAB/DMP is 2:1 and electrophoretic mobility for the liposomes attains a minimum at a positive value. At 5 mM ionic strength, maximal DMP adsorption on the liposome becomes close to zero, demonstrating that the electrostatic attraction essentially drives the DODAB/DMP complexation. Over the millimolar range of DMP concentrations (0.4-1.5 mM), upon nucleotide addition, turbidity of the liposome dispersion (0.08 mM DODAB) steeply increases as a function of time in contrast with the much smaller flocculation rates upon NaCl addition over a much higher range of NaCl concentrations (40-210 mM NaCl). The nucleotide behaves as a hydrophobic anion with an affinity for the membrane that is much higher than that exhibited by a simple anion as chloride. In water, liposome electrophoretic mobility decreases as a function of DMP concentration but the liposome/DMP complex remains positively charged, even at the highest DMP concentrations tested. DMP-induced rupture of liposomes containing [~(14)C]sucrose was evaluated from dialysis of DMP/liposomes mixtures. In water, DMP-induced leakage of radioactive liposomal contents suggests that the DMP/bilayer interaction is not superficial. Although the interaction preserves the positive liposome charge, it does not preserve its integrity. At maximal adsorption, DMP insertion in the cationic bilayer is the most reasonable explanation for the remaining positive charge on the vesicle, the 2:1 DODAB:DMP molar ratio, and leakage of internal contents from the liposome.
机译：描述了2'-脱氧腺苷5'-单磷酸酯（DMP）与水中的二十八烷基二甲基溴化铵（DODAB）组成的阳离子脂质体之间的相互作用。在最大吸附下，DODAB / DMP的摩尔比为2：1，脂质体的电泳迁移率在正值时达到最小值。在5 mM的离子强度下，脂质体上的最大DMP吸附量接近于零，表明静电吸引作用实质上驱动了DODAB / DMP络合。在添加核苷酸后，在DMP浓度的毫摩尔范围（0.4-1.5 mM）内，脂质体分散液的浊度（0.08 mM DODAB）随时间急剧增加，而添加氯化钠后的絮凝率要小得多，而在更高的浓度下NaCl浓度范围（40-210 mM NaCl）。核苷酸表现为疏水性阴离子，对膜的亲和力远高于简单阴离子（如氯离子）所显示的亲和力。在水中，脂质体电泳迁移率随DMP浓度而降低，但脂质体/ DMP复合物即使在测试的最高DMP浓度下仍带正电。通过DMP /脂质体混合物的透析评估DMP诱导的含[〜（14）C]蔗糖的脂质体的破裂。在水中，DMP诱导的放射性脂质体内容物泄漏表明DMP /双层相互作用不是表面现象。尽管相互作用保留了脂质体的正电荷，但没有保留其完整性。在最大吸附下，阳离子双分子层中DMP的插入是囊泡上剩余正电荷，DODAB：DMP摩尔比为2：1以及脂质体内部内容物泄漏的最合理解释。

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