...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomolecular engineering >Phylogenetic relationships and genetic polymorphisms in wild Indian mice
【24h】

Phylogenetic relationships and genetic polymorphisms in wild Indian mice

机译：野生印度印第安小鼠的亲缘关系和遗传多态性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Randomly amplified polymorphic DNA (RAPD) analysis was used to examine the extent of variability in 11 Indian wild derived commensal house mice (Mus musculus) populations and compared with inbred strains of musculus and domesticus subspecies as well as commonly used laboratory inbred strains C57BL/6J and DBA/2J. Arbitrary designed 10 mer oligonucleotide primers with 60-70% (G + C) content were used to amplify DNA template. Out of 52 primers screened initially on the laboratory strains, 20 were selected for analysis on the basis of amplification product in the size range of 200-1400 bp. Among 353 total polymorphic bands, 220 bands (64%) were found to be polymorphic in Indian wild mice, 85 bands (25) in wild derived inbred strains and 37 bands (11%) in laboratory mice strains. The amplification patterns produced by primers were statistically analysed by jaccard's similarity coefficient the value of which ranged from 0.56 to 0.80. High level of genetic diversity was seen in the Indian wild mice populations as compared to the controls. The UPGMA phenogram grouped mice population into two major clusters except Bikaner [BIK], Bilaspur [BIL] and Ranikhet [RK] populations which were placed outside the close-knit clusters. Inspite of low values of bootstrap estimates obtained by Wagner and Dollo parsimony analysis, the results were comparable with UPGMA phenogram when constitution of the populations in the major cluster was considered. Indian mice populations appeared to be diverse from laboratory inbred mice strains.
机译：随机扩增多态性DNA（RAPD）分析用于检查11种印度野生衍生的家鼠（Mus musculus）种群的变异程度，并与小家鼠和家蝇亚种的近交品系以及常用的实验室自交系C57BL / 6J进行比较和DBA / 2J。使用任意设计的含量为60-70％（G + C）的10聚体寡核苷酸引物来扩增DNA模板。在最初从实验室菌株中筛选出的52个引物中，基于200-1400 bp大小的扩增产物，选择了20个进行分析。在353条总多态性条带中，印度野生小鼠中发现220条带（64％），野生近交系中发现85条带（25），实验室小鼠中发现37条带（11％）。用Jaccard的相似系数对引物产生的扩增模式进行统计分析，该相似系数的取值范围为0.56至0.80。与对照组相比，在印度野生小鼠种群中发现了高水平的遗传多样性。 UPGMA象形图将小鼠种群分为两个主要的种群，但Bikaner [BIK]，Bilaspur [BIL]和Ranikhet [RK]种群除外，它们被放置在紧密联系的种群之外。尽管通过Wagner和Dollo简约分析获得的引导程序估计值较低，但当考虑主要群集中的人口构成时，结果与UPGMA象形图相当。印度小鼠种群似乎与实验室近交小鼠菌株不同。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号