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首页> 外文期刊>Biomolecular engineering >Cultivation of explants of the marine sponge Crambe crambe in closed systems
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Cultivation of explants of the marine sponge Crambe crambe in closed systems

机译：封闭系统中海洋海绵Crambe crambe外植体的培养

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Explants of the sponge Crambe crambe were cultured in natural seawater, with or without marine microalga (Phaeodactylum tricornutum) in discontinuous flow through systems and in continuous flow-through systems (DFTHS and CFTHS, respectively). Growth was measured as the increase in underwater weight. In the experiment carried out in the CFTHS, the explants average underwater weight increased by up to 1380% of the initial weight in 22-45 days. Growth in DFTHS was much slower producing a gain of up to an average value of 322% of the initial weight in 100-210 days. Growth kinetics varied considerably for different explants. Explants grew fastest in the first 10-days of subculture. The sponges grew better in CFTHS compared with the DFTHS. The high growth rates observed in CFTHS suggest that this technique is a promising method for culturing C. crambe in closed systems.
机译：海绵Crambe crambe的外植体在有或没有海洋微藻（Phaeodactylum tricornutum）的天然海水中以不连续流过系统和连续流过系统（分别为DFTHS和CFTHS）进行培养。生长量被测量为水下重量的增加。在CFTHS中进行的实验中，外植体的平均水下重量在22-45天内增加了初始重量的1380％。 DFTHS的生长要慢得多，在100-210天内，平均增重可达初始体重的322％。不同外植体的生长动力学差异很大。在亚培养的前10天中，外植体生长最快。与DFTHS相比，CFTHS中的海绵生长更好。在CFTHS中观察到的高生长速率表明，该技术是在封闭系统中培养C. crambe的有前途的方法。

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