...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The journal of physical chemistry, C. Nanomaterials and interfaces >Patterning of Polypeptides on a Collagen-Terminated Tissue Surface
【24h】

Patterning of Polypeptides on a Collagen-Terminated Tissue Surface

机译：胶原蛋白终止的组织表面上的多肽的图案。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Collagen is a ubiquitous component of the extracellular matrix environment, and numerous studies have been devoted toward the development of collagen-based tissue scaffolds. These efforts have been primarily focused on synthetic collagenous materials made from purified collagen. In this article, we present a preliminary study toward the development of a technique that can result in a tissue-derived collagen scaffold. The tissue-derived collagenous matrix was isolated from the retinal Bruch's membrane, and dip pen nanolithography was investigated as a mean to modify the collagenous surface. Characterization experiments of the collagenous surface indicate a fairly hydrophobic surface. Minimal swelling (<7%) of the collagen fibers was observed under elevated humidity conditions with negligible rearrangement of the surface. The hydrophobicity and roughness of the surface can pose a barrier for the deposition of molecules via scanning probe lithography. However, deposition of poly(glutamic acid) and polyarginine onto the surface could be achieved under high contact force and elevated relative humidity conditions (above 55%). Under these conditions, the deposition of the polypeptides occurred through molecular deposition with no observable molecular diffusion. On the other hand, the addition of 0.1% (v/v) Tween-20 surfactant to the inking solution facilitated diffusion of polypeptide inks by increasing the wettability of the collagenous surface. As such, deposition of molecule can be observed under lower contact force at ambient relative humidity (37-40%).
机译：胶原蛋白是细胞外基质环境中普遍存在的成分，并且许多研究致力于基于胶原蛋白的组织支架的开发。这些努力主要集中在由纯化的胶原制成的合成胶原材料上。在本文中，我们对可导致组织衍生的胶原蛋白支架的技术的发展进行了初步研究。从视网膜布鲁赫膜中分离出组织来源的胶原蛋白基质，并研究了浸笔纳米光刻技术作为修饰胶原蛋白表面的手段。胶原表面的表征实验表明相当疏水的表面。在升高的湿度条件下观察到胶原纤维的最小溶胀（<7％），并且表面的重排可以忽略不计。表面的疏水性和粗糙度可为通过扫描探针光刻的分子沉积提供障碍。但是，在高接触力和相对湿度较高（高于55％）的条件下，可以实现聚谷氨酸和聚精氨酸在表面的沉积。在这些条件下，多肽的沉积是通过分子沉积发生的，没有可观察到的分子扩散。另一方面，向着墨溶液中添加0.1％（v / v）的Tween-20表面活性剂通过增加胶原表面的润湿性而促进了多肽油墨的扩散。这样，可以在环境相对湿度（37-40％）下以较低的接触力观察到分子的沉积。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号