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首页> 外文期刊>Chromatographia >HPLC Method for the Dosage of Paclitaxel in Copaiba Oil: Development, Validation, Application to the Determination of the Solubility and Partition Coefficients
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HPLC Method for the Dosage of Paclitaxel in Copaiba Oil: Development, Validation, Application to the Determination of the Solubility and Partition Coefficients

机译：HPLC法测定紫Co油中紫杉醇的用量：研制，验证，在溶解度和分配系数测定中的应用

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摘要

A highly sensitive, simple, rapid, innovative and economic method has been developed and validated for the quantification of paclitaxel in a complex plant matrix, copaiba oil, by HPLC method. Chromatographic separation was performed by Uptisphere Strategy C-18 column and the detection was performed at 228 nm. No interfering peaks from highly complex matrices were observed during the paclitaxel elution. Standard curves of paclitaxel containing 10 mg of copaiba resin oil were linear over the concentration range from 200 to 1700 ng mL(-1) with a determination coefficient of 0.999 and lower regression residues with a homoscedastic dispersion. The lower quantification and detection limits were 33.8 and 10.1 ng mL(-1), respectively. The accuracy and precision determinations were less or equal to 0.60 and 2.03 %, respectively. The method developed was successfully applied to the evaluation of paclitaxel in copaiba resin and essential oils by solubility and partition coefficient studies. Paclitaxel showed a lipophilic characteristic (log P > 1) and a higher solubility in copaiba oils. In conclusion, the method showed sensitivity, linearity, precision, accuracy, necessary specificity and robustness for a reliable separation, identification and quantification of paclitaxel in complex plant matrix. This new analytical method can be, therefore, also applied to quantify paclitaxel in drug delivery systems in which copaiba resin or essential oils are associated, such as lipid and polymer systems.
机译：已经开发了一种高度灵敏，简单，快速，创新和经济的方法，并已通过HPLC方法验证了该方法用于定量复杂植物基质copaiba油中紫杉醇的含量。色谱分离是通过Uptisphere Strategy C-18色谱柱进行的，检测是在228 nm进行的。在紫杉醇洗脱过程中未观察到来自高度复杂基质的干扰峰。在200至1700 ng mL（-1）的浓度范围内，含10 mg copaiba树脂油的紫杉醇的标准曲线呈线性，测定系数为0.999，回归残渣较低，具有同调分散性。较低的定量和检测极限分别为33.8和10.1 ng mL（-1）。准确度和精密度测定分别小于或等于0.60和2.03％。通过溶解度和分配系数研究，所开发的方法已成功地用于评估copaiba树脂和香精油中的紫杉醇。紫杉醇表现出亲脂性（log P> 1），并在copaiba油中具有更高的溶解度。总之，该方法显示了灵敏度，线性，精密度，准确性，必要的特异性和鲁棒性，可用于复杂植物基质中紫杉醇的可靠分离，鉴定和定量。因此，这种新的分析方法还可用于定量与copaiba树脂或香精油结合的药物输送系统（例如脂质和聚合物系统）中的紫杉醇。

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