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Protein and peptide arrays: recent trends and new directions.

机译:蛋白质和多肽阵列:最新趋势和新方向。

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摘要

Microarrays of proteins and peptides make it possible the screening of thousands of binding events in a parallel and high throughput fashion; therefore they are emerging as a powerful tool for proteomics and clinical assays. The complex nature of Proteome, the wide dynamic range of protein concentration in real samples and the critical role of immobilized protein orientation must be taken into account to maximize the utility of protein microarrays. Immobilization strategy and designing of an ideal local chemical environment on the solid surface are both essential for the success of a protein microarray experiment. This review article will focus on protein and peptide arrays highlighting their technical challenges and presenting new directions by means of a set of selected recent applications.
机译:蛋白质和多肽的微阵列使以平行和高通量的方式筛选成千上万个结合事件成为可能。因此,它们正在成为蛋白质组学和临床检测的强大工具。蛋白质组的复杂性质,实际样品中蛋白质浓度的宽动态范围以及固定化蛋白质取向的关键作用必须加以考虑,以最大程度地利用蛋白质微阵列。固定策略和在固体表面上理想的局部化学环境的设计对于蛋白质微阵列实验的成功都是至关重要的。本文将重点关注蛋白质和多肽阵列,重点介绍其技术挑战并通过一系列精选的近期应用提出新的方向。

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