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首页> 外文期刊>The Journal of Nutritional Biochemistry >Dietary extra virgin olive oil polyphenols supplementation modulates DSS-induced chronic colitis in mice.
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Dietary extra virgin olive oil polyphenols supplementation modulates DSS-induced chronic colitis in mice.

机译：膳食特级初榨橄榄油多酚补充剂可调节DSS诱导的小鼠慢性结肠炎。

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摘要

We evaluated the protective effect of dietary extra virgin olive oil (EVOO) polyphenol extract (PE) supplementation in the inflammatory response associated to chronic colitis model. Six-week-old mice were randomized in four dietary groups: standard diet (SD), EVOO diet and both enriched with PE (850 ppm) (SD+PE and EVOO+PE). After 30 days, animals that were exposed to dextran sodium sulfate (DSS) (3%) followed by 3 weeks of drinking water developed chronic colitis, which was evaluated by disease activity index (DAI) and histology. Cell proliferation was analyzed by immunohistochemical and changes in monocyte chemotactic protein (MCP)-1 and tumor necrosis factor (TNF)- alpha mRNA expression by quantitative real-time polymerase chain reaction. Colonic expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, mitogen-activated protein kinases (MAPKs), I kappaB alpha inhibitory and peroxisome proliferator-activated receptor gamma (PPAR gamma) were determined by western blotting. SD-DSS group showed a significant increase of DAI, histological damage and cell proliferation, as well as an up-regulation of TNF- alpha, MCP-1, COX-2 and iNOS proteins. p38 and JNK MAPKs phosphorylation, I kappaB alpha degradation and PPAR gamma deactivation were also observed. However, in DSS-treated and EVOO+PE-fed mice, DAI and cell proliferation were significantly reduced, as well as MCP-1, TNF- alpha, COX-2 and iNOS expression levels. In addition, this dietary group, notably down-regulated JNK phosphorylation, prevented I kappaB alpha degradation and PPAR gamma deactivation. These results demonstrated, for the first time, that EVOO-PE supplementation possessed marked protective effects on experimental colitis through PPAR gamma up-regulation and nuclear transcription factor-kappa B and MAPK signaling pathway inhibition, decreasing the inflammatory cascade. We concluded that PE-enriched EVOO diet could be a beneficial functional food on ulcerative colitis
机译：我们评估了膳食特级初榨橄榄油（EVOO）多酚提取物（PE）补充剂在与慢性结肠炎模型相关的炎症反应中的保护作用。将六周龄的小鼠随机分为四个饮食组：标准饮食（SD），EVOO饮食，并且都富含PE（850 ppm）（SD + PE和EVOO + PE）。 30天后，暴露于葡聚糖硫酸钠（DSS）（3％），然后喝水3周的动物发展为慢性结肠炎，可通过疾病活动指数（DAI）和组织学对其进行评估。通过免疫组织化学分析细胞增殖，并通过定量实时聚合酶链反应分析单核细胞趋化蛋白（MCP）-1和肿瘤坏死因子（TNF）-αmRNA表达的变化。通过蛋白质印迹法确定了诱导型一氧化氮合酶（iNOS），环氧合酶（COX）-2，促分裂原激活的蛋白激酶（MAPK），IκB抑制和过氧化物酶体增殖物激活的受体γ（PPAR gamma）的结肠表达。 SD-DSS组显示DAI显着增加，组织学损伤和细胞增殖，以及TNF-α，MCP-1，COX-2和iNOS蛋白的上调。还观察到p38和JNK MAPK磷酸化，I kappaBα降解和PPARγ失活。但是，在用DSS治疗和EVOO + PE喂养的小鼠中，DAI和细胞增殖以及MCP-1，TNF-α，COX-2和iNOS表达水平明显降低。此外，该饮食组，特别是下调了JNK的磷酸化，防止了I kappaBα降解和PPARγ失活。这些结果首次证明，EVOO-PE补充剂通过PPARγ上调和核转录因子-κB和MAPK信号通路抑制，对实验性结肠炎具有显着的保护作用，从而减少了炎症级联反应。我们得出结论，富含PE的EVOO饮食可能是治疗溃疡性结肠炎的有益功能食品

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