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首页> 外文期刊>Biomolecular engineering >DNA chip replication for a personalized DNA chip.
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DNA chip replication for a personalized DNA chip.

机译：DNA芯片复制用于个性化DNA芯片。

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摘要

We report the replication technology of DNA chip using by sequence specific localization of nucleic acids via hybridization and electric transfer of the nucleic acids onto a new substrate without losing their array information. The denatured DNA fragments are first spotted and UV-cross-linked on a nylon membrane. The membrane is then immersed and hybridized in a DNA mixture solution that contains all complementary sequences of the nucleic acids to be hybridized with the DNA fragments on the membrane. The hybridized DNA fragments are transferred to another membrane at the denatured condition. After separating two membranes, the transferred membrane contains a complementary array of DNA fragments. This method can be used for the replication of the same copy of DNA chip repeatedly and moreover could be applied for a personalized DNA chip fabrication, where specific information of each spot of DNA chip is originated from the genetic information of a personal sample.
机译：我们报告了DNA芯片的复制技术，该技术通过对核酸进行杂交和电转移到新的底物上而不会丢失其阵列信息，通过核酸的序列特异性定位来使用。首先将变性的DNA片段点样并在尼龙膜上进行UV交联。然后将膜浸入DNA混合物溶液中并与其杂交，该DNA混合物溶液包含待与膜上DNA片段杂交的所有核酸互补序列。在变性条件下，杂交的DNA片段被转移到另一膜上。分离两个膜后，转移的膜包含DNA片段的互补阵列。该方法可用于重复复制相同副本的DNA芯片，而且还可用于个性化DNA芯片制造，其中DNA芯片每个点的特定信息均来自个人样品的遗传信息。

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