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首页> 外文期刊>The Journal of Nutritional Biochemistry >GC-TOF-MS-based serum metabolomic investigations of naked oat bran supplementation in high-fat-diet-induced dyslipidemic rats
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GC-TOF-MS-based serum metabolomic investigations of naked oat bran supplementation in high-fat-diet-induced dyslipidemic rats

机译：基于GC-TOF-MS的高脂饮食诱导的血脂异常大鼠补充裸麦麸的血清代谢组学研究

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The present study aimed to explore the metabolic response of oat bran consumption in dyslipidemic rats by a high-throughput metabolomics approach. Four groups of Sprague-Dawley rats were used: N group (normal chow diet), M group (dyslipidemia induced by 4-week high-fat feeding, then normal chow diet), OL group and OH group (dyslipidemia induced, then normal chow diet supplemented with 10.8% or 43.4% naked oat bran). Intervention lasted for 12 weeks. Gas chromatography quadrupole time-of-flight mass spectrometry was used to identify serum metabolite profiles. Results confirmed the effects of oat bran on improving lipidemic variables and showed distinct metabolomic profiles associated with diet intervention. A number of endogenous molecules were changed by high-fat diet and normalized following supplementation of naked oat bran. Elevated levels of serum unsaturated fatty acids including arachidonic acid (Log2Fold of change=0.70, P=.02 OH vs. M group), palmitoleic acid (Log2Fold of change-=1.24, P=.02 OH vs. M group) and oleic acid (Log2Fold of change=0.66, P=.04 OH vs. M group) were detected after oat bran consumption. Furthermore, consumption of oat bran was also characterized by higher levels of methionine and S-adenosylmethionine. Pathway exploration found that most of the discriminant metabolites were involved in fatty acid biosynthesis, biosynthesis and metabolism of amino acids, microbial metabolism in diverse environments and biosynthesis of plant secondary metabolites. These results point to potential biomarkers and underlying benefit of naked oat bran in the context of diet-induced dyslipidemia and offer some insights into the mechanism exploration. (C) 2015 Elsevier Inc. All rights reserved.
机译：本研究旨在通过高通量代谢组学方法探讨血脂异常大鼠中燕麦麸的代谢反应。使用四组Sprague-Dawley大鼠：N组（正常饮食），M组（4周高脂喂养引起的血脂异常，然后正常饮食），OL组和OH组（血脂异常，然后正常饮食）饮食中补充了10.8％或43.4％的裸燕麦麸）。干预持续了12周。气相色谱四极杆飞行时间质谱用于鉴定血清代谢物谱。结果证实了麦麸对改善血脂水平的影响，并显示了与饮食干预相关的独特的代谢组学特征。高脂饮食改变了许多内源性分子，并在补充了裸麦麸后正常化。血清不饱和脂肪酸水平升高，包括花生四烯酸（Log2Fold的变化= 0.70，P = .02 OH vs. M组），棕榈油酸（Log2Fold的变化-= 1.24，P = .02 OH vs. M组）和油酸食用燕麦麸后检测到酸（Log2Fold变化量= 0.66，P = .04 OH vs. M组）。此外，燕麦麸的消费还具有较高水平的蛋氨酸和S-腺苷蛋氨酸的特征。途径探索发现，大多数判别代谢物都参与脂肪酸的生物合成，氨基酸的生物合成和代谢，多种环境中的微生物代谢以及植物次生代谢物的生物合成。这些结果指出了在饮食诱发的血脂异常的情况下裸燕麦麸的潜在生物标志物和潜在的益处，并为机理探索提供了一些见识。 （C）2015 Elsevier Inc.保留所有权利。

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