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首页> 外文期刊>The Journal of Nutritional Biochemistry >Supplementation with vitamins C and E improves mouse lung repair.
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Supplementation with vitamins C and E improves mouse lung repair.

机译：补充维生素C和E可改善小鼠肺部修复。

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Cigarette smoke (CS) induces emphysema by tissue destruction through the production of oxidants and metalloproteinases [matrix metalloproteinases (MMPs)]. The possibility of lung repair after emphysema remains unclear. Our aim was to study the effects of vitamins C and E on mouse lung repair evaluated by catalase (CAT), superoxide dismutase (SOD) and MMP-9 activities; by the amount of tumor necrosis factor (TNF)-alpha in lung homogenates; by cell quantification in bronchoalveolar lavage (BAL) fluid; and by lung histology. Male C57BL/6 mice (n=25) were exposed to nine cigarettes per day, 7 days/week, for 60 days in a whole-body exposure chamber. The control group was sham smoked (n=10). After 60 days of CS exposure, a group of animals was sacrificed (n=5) and the others were divided into two groups: (a) CSv (n=10) supplemented with saline and olive oil (vehicles) for 60 days and (b) CSr (n=10) supplemented with vitamins C and E (50 mg/kg/day) for 60 days. These mice were then sacrificed; BAL was performed and the lungs were removed for biochemical and histological analysis. The results demonstrated that CAT activity was decreased in the CSv and CSr groups compared to the control group. SOD activity was higher in the CSv group than in the control and CSr groups. The CSv group showed a higher neutrophil count in BAL fluid, associated with more TNF-alpha in lung homogenates, than the control or CSr groups. Finally, emphysema in the CSv group was associated with fewer collagen and elastic fibers than in the control and CSr groups. These results indicate a possible role of vitamins C and E in lung repair after emphysema induced by long-term CS exposure in mice.
机译：香烟烟雾（CS）通过产生氧化剂和金属蛋白酶[基质金属蛋白酶（MMP）]破坏组织，从而诱发肺气肿。肺气肿后肺修复的可能性仍不清楚。我们的目的是研究过氧化氢酶（CAT），超氧化物歧化酶（SOD）和MMP-9活性评估的维生素C和E对小鼠肺修复的影响;通过肺匀浆中肿瘤坏死因子（TNF）-α的量;通过支气管肺泡灌洗液（BAL）中的细胞定量;并通过肺组织学检查。将雄性C57BL / 6小鼠（n = 25）每天在全身暴露室内每天暴露9支烟，每周7天，每天60天。对照组为假烟（n = 10）。接触CS 60天后，处死一组动物（n = 5），将其他动物分为两组：（a）补充生理盐水和橄榄油（车辆）的CSv（n = 10）60天，和（ b）CSr（n = 10）补充维生素C和E（50 mg / kg /天）60天。然后处死这些小鼠。进行BAL，取出肺进行生化和组织学分析。结果表明，与对照组相比，CSv和CSr组的CAT活性降低。 CSv组的SOD活性高于对照组和CSr组。与对照组或CSr组相比，CSv组在BAL液中显示出更高的中性粒细胞计数，并与肺匀浆中的更多TNF-α相关。最后，与对照组和CSr组相比，CSv组的肺气肿与较少的胶原蛋白和弹性纤维有关。这些结果表明，维生素C和E在长期CS引起的小鼠肺气肿后肺修复中可能发挥作用。

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