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首页> 外文期刊>The Journal of Nutritional Biochemistry >Impaired translation initiation activation and reduced protein synthesis in weaned piglets fed a low-protein diet.
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Impaired translation initiation activation and reduced protein synthesis in weaned piglets fed a low-protein diet.

机译：低蛋白饮食断奶仔猪的翻译起始激活受损，蛋白质合成减少。

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Weanling mammals (including infants) often experience intestinal dysfunction when fed a high-protein diet. Recent work with the piglet (an animal model for studying human infant nutrition) shows that reducing protein intake can improve gut function during weaning but compromises the provision of essential amino acids (EAA) for muscle growth. The present study was conducted with weaned pigs to test the hypothesis that supplementing deficient EAA (Lys, Met, Thr, Trp, Leu, Ile and Val) to a low-protein diet may maintain the activation of translation initiation factors and adequate protein synthesis in tissues. Pigs were weaned at 21 days of age and fed diets containing 20.7, 16.7 or 12.7% crude protein (CP), with the low-CP diets supplemented with EAA to achieve the levels in the high-CP diet. On Day 14 of the trial, tissue protein synthesis was determined using the phenylalanine flooding dose method. Reducing dietary CP levels decreased protein synthesis in pancreas, liver, kidney and longissimus muscle. A low-CP diet reduced the phosphorylation of eukaryotic initiation factor (eIF) 4E-binding protein-1 (4E-BP1) in skeletal muscle and liver while increasing the formation of an inactive eIF4E.4E-BP1 complex in muscle. Dietary protein deficiency also decreased the phosphorylation of mammalian target of rapamycin (mTOR) and the formation of an active eIF4E.eIF4G complex in liver. These results demonstrate for the first time that chronic feeding of a low-CP diet suppresses protein synthesis in animals partly by inhibiting mTOR signaling. Additionally, our findings indicate that supplementing deficient EAA to low-protein diets is not highly effective in restoring protein synthesis or whole-body growth in piglets. We suggest that conditionally essential amino acids (e.g., glutamine and arginine) may be required to maintain the activation of translation initiation factors and optimal protein synthesis in neonates
机译：断奶的哺乳动物（包括婴儿）在进食高蛋白饮食时通常会出现肠道功能障碍。仔猪（用于研究人类婴儿营养的动物模型）的最新研究表明，减少蛋白质摄入量可以改善断奶期间的肠道功能，但会损害肌肉生长所必需的氨基酸（EAA）的供应。本研究是对断奶的猪进行的，以检验以下假设：低蛋白饮食补充不足的EAA（Lys，Met，Thr，Trp，Leu，Ile和Val）可以维持翻译起始因子的激活和适当的蛋白质合成。组织。猪在21日龄时断奶，并饲喂含有20.7、16.7或12.7％粗蛋白（CP）的日粮，低CP日粮补充了EAA以达到高CP日粮的水平。在试验的第14天，使用苯丙氨酸淹没剂量法确定组织蛋白的合成。降低饮食中CP水平会降低胰腺，肝脏，肾脏和最长肌的蛋白质合成。低CP饮食可减少骨骼肌和肝脏中真核起始因子（eIF）4E结合蛋白1（4E-BP1）的磷酸化，同时增加肌肉中无活性的eIF4E.4E-BP1复合物的形成。饮食中蛋白质的缺乏还降低了哺乳动物雷帕霉素靶标（mTOR）的磷酸化和肝脏中活性eIF4E.eIF4G复合物的形成。这些结果首次证明，长期饲喂低CP饮食可部分抑制mTOR信号传导，从而抑制动物体内的蛋白质合成。此外，我们的研究结果表明，向低蛋白饮食补充不足的EAA对恢复仔猪的蛋白质合成或全身生长不是十分有效。我们建议可能需要有条件的必需氨基酸（例如谷氨酰胺和精氨酸）来维持新生儿翻译起始因子的激活和最佳蛋白质合成

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