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首页> 外文期刊>The Journal of Nutritional Biochemistry >Dietary supplementation with zinc oxide stimulates ghrelin secretion from the stomach of young pigs
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Dietary supplementation with zinc oxide stimulates ghrelin secretion from the stomach of young pigs

机译：膳食补充氧化锌可刺激幼猪胃中的生长素释放肽分泌

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Dietary supplementation with zinc is known to enhance food intake and growth in young children. However, the underlying mechanisms remain largely unknown. Ghrelin, a peptide derived mainly from stomach, plays an important role in food-intake regulation. The present study was conducted with the piglet model to test the hypothesis that zinc may increase gastric ghrelin secretion. In Experiment 1 (Exp. 1) , thirty-six 28-day- old weaned pigs were assigned to two groups (18 pigs/group), receiving four-week supplementation of 0 or 2000 mg/kg Zn (as ZnO) to the basal diet containing 100 mg/kg Zn. In Experiment (Exp. 2), sixteen 28-day-old piglets were assigned to the same treatments (n=8/group) as in Exp. 1, except that they were pair-fed an equal amount of diet. At the end of the experiments, blood, stomach and duodenum samples were obtained for biochemical analysis, including assays of ghrelin protein and insulin-like growth factor-I (IGF-I) in plasma, as well as quantification of ghrelin and IGF-I mRNA levels in the duodenum and gastric mucosa. Further, gastric mucosal cells from unsupplemented piglets were cultured with 0-0.5 mM ZnO for 2-24 h for assays of ghrelin production and gene expression. Dietary Zn supplementation increased plasma concentrations of ghrelin, IGF-I and cholecystokinin; IGF-I gene expression in the duodenum as well as food intake and piglet growth (Exp. 1). The effects of ZnO on plasma levels of ghrelin, intestinal IGF-I expression and piglet growth were independent of food intake. Addition of ZnO to culture medium enhanced ghrelin production from gastric mucosal cells without affecting ghrelin mRNA levels. Collectively, our results indicate that ZnO stimulates ghrelin secretion from the stomach at the post-transcriptional level. This novel finding aids in elucidating the cellular and molecular mechanism for a role of zinc in promoting food intake and growth of young children.
机译：众所周知，膳食补充锌可以增加幼儿的食物摄入和生长。但是，基本机制仍然未知。 Ghrelin是一种主要来源于胃的肽，在食物摄入调节中起着重要作用。本研究是用仔猪模型进行的，以检验锌可能增加胃饥饿素分泌的假设。在实验1（实验1）中，将36只28天大的断奶猪分为两组（每组18头猪），并接受四周的0或2000 mg / kg Zn（以ZnO形式）补充。基础饮食中含锌100 mg / kg。在实验（实验2）中，将16只28天大的仔猪分配给与实验2相同的处理（n = 8 /组）。 1，除了他们被配对喂等量的饮食。在实验结束时，获得了血液，胃和十二指肠样品用于生化分析，包括血浆中生长素释放肽蛋白和胰岛素样生长因子-I（IGF-I）的测定，以及生长素释放肽和IGF-I的定量十二指肠和胃粘膜中的mRNA水平。此外，将未补充的仔猪的胃粘膜细胞与0-0.5 mM ZnO培养2-24小时，以检测生长素释放肽的产生和基因表达。膳食锌补充增加了生长素释放肽，IGF-I和胆囊收缩素的血浆浓度;十二指肠中的IG​​F-I基因表达以及食物摄入和仔猪生长（实验1）。 ZnO对生长素释放肽血浆水平，肠道IGF-I表达和仔猪生长的影响与食物摄入无关。向培养基中添加ZnO可增强胃粘膜细胞中生长素释放肽的产生，而不会影响生长素释放肽mRNA的水平。总体而言，我们的结果表明，ZnO在转录后水平上刺激胃中ghrelin的分泌。这一新发现有助于阐明锌在促进幼儿食物摄入和生长中的作用的细胞和分子机制。

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