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首页> 外文期刊>Biomolecular engineering >Real-time study of the effect of different stress factors on lactic acid bacteria by electrochemical optical waveguide lightmode spectroscopy.
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Real-time study of the effect of different stress factors on lactic acid bacteria by electrochemical optical waveguide lightmode spectroscopy.

机译：通过电化学光波导光模光谱实时研究不同应力因子对乳酸菌的影响。

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摘要

Lactic acid bacteria play an important role in the fermentation of different food products. During the fermentation processes, lactobacilli are confronted with many inhibitor factors. These factors by themselves or in combination can influence the growth of lactic acid bacteria and their acidification capacity. The subject of our study was to monitor with a newly developed biosensing technique how the different chemical stress factors influence the survival of lactic acid bacteria. Electrochemical optical waveguide lightmode spectroscopy combines evanescent-field optical sensing with electrochemical control of surface adsorption processes. For optical sensing, a layer of indium tin oxide served as a high refractive index waveguide and as a conductive electrode, as well. Lactobacillus plantarum 2142 suspended in Jerusalem artichoke syrup was used in the experiments. Electrochemical optical waveguide lightmode spectroscopy measurements were undertaken by using OW 2400c indium tin oxide coated waveguide sensors (MicroVacuum, Budapest, Hungary) and were performed in a flow-injection analyzer system. The bacterial cells were adsorbed in native form without any chemical binding on the surface of the sensor by ensuring polarizing potential (1V) and were exposed to different concentration of acetic acid/Jerusalem artichoke syrup, lactic acid/Jerusalem artichoke syrup and hydrogen peroxide/Jerusalem artichoke syrup solution for 1h, respectively, and the effect on bacteria cells was monitored. Results were compared to the traditional micro-assay method, and it can be assumed that after further investigations this new technique could be used in real-time application.
机译：乳酸菌在不同食品的发酵中起重要作用。在发酵过程中，乳杆菌面临许多抑制因子。这些因素本身或结合起来可影响乳酸菌的生长及其酸化能力。我们的研究主题是用新开发的生物传感技术监测不同的化学应激因素如何影响乳酸菌的存活。电化学光波导光模光谱将瞬逝场光学传感与表面吸附过程的电化学控制相结合。对于光学感测，铟锡氧化物层还用作高折射率波导和导电电极。实验中使用了悬浮在菊芋糖浆中的植物乳杆菌2142。使用OW 2400c氧化铟锡涂层的波导传感器（MicroVacuum，布达佩斯，匈牙利）进行电化学光波导光模光谱测量，并在流动注射分析仪系统中进行。通过确保极化电位（1V），细菌细胞以天然形式被吸附，没有任何化学键结合在传感器表面，并暴露于不同浓度的乙酸/菊芋糖浆，乳酸/菊芋糖浆和过氧化氢/耶路撒冷中朝鲜蓟糖浆溶液分别放置1h，并监测其对细菌细胞的影响。将结果与传统的微量测定方法进行了比较，可以假设，经过进一步的研究，可以将该新技术用于实时应用。

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