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首页> 外文期刊>The Journal of Nutritional Biochemistry >Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids.
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Lipid alterations in human colon epithelial cells induced to differentiation and/or apoptosis by butyrate and polyunsaturated fatty acids.

机译：丁酸酯和多不饱和脂肪酸可诱导人结肠上皮细胞的脂质变化诱导分化和/或凋亡。

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摘要

The present study highlights the important association between lipid alterations and differentiation/apoptotic responses in human colon differentiating (FHC) and nondifferentiating (HCT-116) cell lines after their treatment with short-chain fatty acid sodium butyrate (NaBt), polyunsaturated fatty acids (PUFAs), and/or their combination. Our data from GC/MS and LC/MS/MS showed an effective incorporation and metabolization of the supplemented arachidonic acid (AA) or docosahexaenoic acid (DHA), resulting in an enhanced content of the respective PUFA in individual phospholipid (PL) classes and an altered composition of the whole cellular fatty acid spectrum in both FHC and HCT-116 cells. We provide novel evidence that NaBt combined with PUFAs additionally modulated AA and DHA cellular levels and caused their shift from triacylglycerol to PL fractions. NaBt increased, while AA, DHA and their combination with NaBt decreased endogenous fatty acid synthesis in FHC but not in HCT-116 cells. Fatty acid treatment also altered membrane lipid structure, augmented cytoplasmic lipid droplet accumulation, reactive oxygen species (ROS) production and dissipation of the mitochondrial membrane potential. All these parameters were significantly enhanced by combined NaBt/PUFA treatment, but only in FHC cells was this accompanied by highly increased apoptosis and suppressed differentiation. Moreover, the most significant changes of ROS production, differentiation and apoptosis among the parameters studied, the highest effects of combined NaBt/PUFA treatment and a lower sensitivity of HCT-116 cells were confirmed using two-way ANOVA. Our results demonstrate an important role of fatty acid-induced lipid alterations in the different apoptotic/differentiation response of colon cells with various carcinogenic potential
机译：本研究着重介绍了短链脂肪酸丁酸钠（NaBt），多不饱和脂肪酸（NaHt）处理后，人结肠分化（FHC）和非分化（HCT-116）细胞系中脂质变化与分化/凋亡反应之间的重要关联。 PUFA）和/或它们的组合。我们从GC / MS和LC / MS / MS获得的数据表明，补充的花生四烯酸（AA）或二十二碳六烯酸（DHA）的有效掺入和代谢，导致各个磷脂（PL）类中各个PUFA的含量增加，并且FHC和HCT-116细胞中整个细胞脂肪酸谱的组成发生了变化。我们提供了新颖的证据，表明NaBt与PUFA组合还可以调节AA和DHA细胞水平，并导致其从三酰基甘油转移到PL组分。 NaBt增加，而AA，DHA及其与NaBt的结合减少FHC中的内源性脂肪酸合成，而在HCT-116细胞中则没有。脂肪酸处理还改变了膜脂质结构，增加了细胞质脂质滴的积累，增加了活性氧（ROS）的产生和线粒体膜电位的耗散。 NaBt / PUFA联合处理可显着提高所有这些参数，但仅在FHC细胞中，这会伴随高度凋亡和抑制分化。此外，使用双向方差分析证实了所研究的参数中ROS产生，分化和凋亡的最显着变化，NaBt / PUFA联合治疗的最高效果和HCT-116细胞的较低敏感性。我们的结果证明了脂肪酸诱导的脂质改变在具有各种致癌潜能的结肠细胞的不同凋亡/分化反应中的重要作用

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