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首页> 外文期刊>The Journal of Nutritional Biochemistry >Zebrafish (Danio rerio) fed vitamin E-deficient diets produce embryos with increased morphologic abnormalities and mortality.
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Zebrafish (Danio rerio) fed vitamin E-deficient diets produce embryos with increased morphologic abnormalities and mortality.

机译：维生素E缺乏饮食喂养的斑马鱼（ Danio rerio ）会产生形态异常和死亡率增加的胚胎。

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Vitamin E ( alpha-tocopherol) is required to prevent fetal resorption in rodents. To study alpha-tocopherol's role in fetal development, a nonplacental model is required. Therefore, the zebrafish, an established developmental model organism, was studied by feeding the fish a defined diet with or without added alpha-tocopherol. Zebrafish (age, 4-6 weeks) were fed the deficient (E--), sufficient (E+) or lab diet up to 1 years. All groups showed similar growth rates. The exponential rate of alpha-tocopherol depletion up to approx. equal to 80 day in E-- zebrafish was 0.029 +or- 0.006 nmol/g, equivalent to a depletion half-life of 25 +or- 5 days. From age approx. equal to 80 days, the E-- fish (5 +or- 3 nmol/g) contained approx. equal to 50 times less alpha-tocopherol than the E+ or lab diet fish (369 +or- 131 or 362 +or- 107, respectively; P<.05). E-depleted adults demonstrated decreased startle response suggesting neurologic deficits. Expression of selected oxidative stress and apoptosis genes from livers isolated from the zebrafish fed the three diets were evaluated by quantitative polymerase chain reaction and were not found to vary with vitamin E status. When E-depleted adults were spawned, they produced viable embryos with depleted alpha-tocopherol concentrations. The E-- embryos exhibited a higher mortality (P<.05) at 24 h post-fertillization and a higher combination of malformations and mortality (P<.05) at 120 h post-fertillization than embryos from parents fed E+ or lab diets. This study documents for the first time that vitamin E is essential for normal zebrafish embryonic development
机译：需要维生素E（α-生育酚）来防止胎儿在啮齿动物中吸收。为了研究α-生育酚在胎儿发育中的作用，需要非胎盘模型。因此，斑马鱼是一种已建立的发育模型生物，通过给鱼类喂食确定的饮食（添加或不添加α-生育酚）来进行研究。斑马鱼（4-6周龄）的食物不足（E--），充足（E +）或实验室饮食长达1年。所有组均显示相似的增长率。 α-生育酚消耗的指数速率高达在E-斑马鱼中等于80天的等于0.029±0.006 nmol / g，相当于25±5天的耗竭半衰期。从年龄开始等于80天，E--鱼（5±3 nmol / g）约含等于E +或实验室饮食鱼的α-生育酚含量低50倍（分别为369 + or- 131或362 + or-107; P <.05）。电子贫乏的成年人表现出惊吓反应减少，提示神经功能缺损。通过定量聚合酶链反应评估了从饲喂三种饮食的斑马鱼中分离出的肝脏中选定的氧化应激和凋亡基因的表达，未发现其随维生素E状态的变化而变化。当产生E耗尽的成虫时，它们产生的Al-生育酚浓度降低的活胚。与受E +或实验室饮食喂养的父母的胚胎相比，受精后24 h的E--胚胎死亡率更高（P <.05），受精后120 h畸形和死亡率更高（P <.05）。 。这项研究首次证明维生素E对正常斑马鱼的胚胎发育至关重要

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