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首页> 外文期刊>The Journal of Nutritional Biochemistry >Effects of long-term consumption of low doses of resveratrol on diet-induced mild hypercholesterolemia in pigs: a transcriptomic approach to disease prevention.
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Effects of long-term consumption of low doses of resveratrol on diet-induced mild hypercholesterolemia in pigs: a transcriptomic approach to disease prevention.

机译：长期低剂量白藜芦醇的摄入对饮食中猪引起的轻度高胆固醇血症的影响：一种预防疾病的转录组学方法。

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摘要

Metabolic and cardiovascular diseases (CVDs) have risen to alarming proportions, and there is a need for therapeutic and preventive measures. The polyphenol resveratrol (RES) protects against CVDs, but in vivo molecular mechanisms responsible for protection are not yet understood. Peripheral blood mononuclear cells (PBMNCs) are involved in the development of atherosclerosis and metabolic disorders. The identification of PBMNCs genes responding to dietary compounds might help to understand the mechanisms underlying the effects of polyphenols. We determined gene expression differences between PBMNCs from pigs fed a high-fat diet manifesting a mild increase of cholesterol and pigs fed a high-fat diet containing low doses of RES. Although the consumption of RES did not modify the levels of cholesterol, microarray analyses indicated that some of the differentially expressed genes, collagens (COL1A, COL3A), lipoprotein lipase (LPL) and fatty-acid binding proteins (FABPs) involved in CVDs and lipid metabolism were up-regulated by the high-fat diet and down-regulated by RES. Reverse transcriptase polymerase chain reaction confirmed that RES and RES-containing grape extract prevented the induction of FABP4 in PBMNCs in female pigs fed a high-fat diet. Low micromolar concentrations of RES and its metabolite dihydroresveratrol exerted a minor but significant reducing effect on the induction of FABP4 expression in human macrophages treated with oxidized low-density lipoprotein. Our results show that the consumption of low doses of RES modulates the expression of genes related to lipid metabolism and metabolic disorders that are affected by a high-fat diet and suggest that some of the circulating RES metabolites may contribute to these effects
机译：代谢和心血管疾病（CVD）的比例已达到令人震惊的程度，因此需要采取治疗和预防措施。多酚白藜芦醇（RES）可以防止CVD，但是尚不了解负责保护作用的体内分子机制。外周血单核细胞（PBMNC）参与动脉粥样硬化和代谢性疾病的发展。鉴定对膳食化合物有反应的PBMNCs基因可能有助于了解多酚作用的潜在机制。我们确定了饲喂高脂饮食显示胆固醇轻度升高的猪和饲喂高脂饮食包含低剂量RES的猪之间的PBMNCs的基因表达差异。尽管食用RES不会改变胆固醇水平，但微阵列分析表明，一些差异表达的基因，胶原蛋白（COL1A，COL3A），脂蛋白脂肪酶（LPL）和脂肪酸结合蛋白（FABP）与CVD和脂质有关高脂饮食上调新陈代谢，而RES下调新陈代谢。逆转录酶聚合酶链反应证实，RES和含RES的葡萄提取物可以阻止高脂饮食雌性猪PBMNCs中FABP4的诱导。低微摩尔浓度的RES及其代谢物二氢白藜芦醇对用氧化型低密度脂蛋白处理的人巨噬细胞中FABP4表达的诱导产生较小但明显的降低作用。我们的结果表明，低剂量RES的消耗可调节与受高脂饮食影响的脂质代谢和代谢紊乱有关的基因的表达，并表明某些循环中的RES代谢产物可能有助于这些作用

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