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首页> 外文期刊>The Journal of Nutritional Biochemistry >High-protein diet differently modifies intestinal goblet cell characteristics and mucosal cytokine expression in ileum and colon
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High-protein diet differently modifies intestinal goblet cell characteristics and mucosal cytokine expression in ileum and colon

机译：高蛋白饮食会不同地改变回肠和结肠中的肠道杯状细胞特征和黏膜细胞因子表达

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We have previously shown that high-protein (HP) diet ingestion causes marked changes in the luminal environment of the colonic epithelium. This study aimed to evaluate the impact of such modifications on small intestinal and colonic mucosa, two segments with different transit time and physiological functions. Rats were fed with either normal protein (NP; 14% protein) or HP (53% protein) isocaloric diet for 2 weeks, and parameters related to intestinal mucous-secreting cells and to several innate/adaptive immune characteristics (myeloperoxidase activity, cytokine and epithelial TLR expression, proportion of immune cells in gut-associated lymphoid tissues) were measured in the ileum and colon. In ileum from HP animals, we observed hyperplasia of mucus-producing cells concomitant with an increased expression of Muc2 at both gene and protein levels, reduction of mucosal myeloperoxidase activity, down-regulation of TIr4 gene expression in enterocytes and down-regulation of mucosal Th cytokines associated with CD4+ lymphocyte reduction in mesenteric lymph nodes. These changes coincided with an increased amount of acetate in the ileal luminal content. In colon, HP diet ingestion resulted in a lower number of goblet cells at the epithelial surface but increased goblet cell number in colonic crypts together with an increased Muc3 and a slight reduction of 11-6 gene expression. Our data suggest that HP diet modifies the goblet cell distribution in colon and, in ileum, increases goblet cell activity and decreases parameters related to basal gut inflammatory status. The impact of HP diet on intestinal mucosa in terms of beneficial or deleterious effects is discussed. (C) 2015 Elsevier Inc. All rights reserved.
机译：先前我们已经表明，高蛋白（HP）饮食摄入会引起结肠上皮腔环境的明显变化。这项研究旨在评估这种修饰对小肠和结肠粘膜的影响，小肠和结肠粘膜具有两个不同的转运时间和生理功能。用正常蛋白质（NP; 14％蛋白质）或HP（53％蛋白质）等热量饮食喂养大鼠2周，其参数与肠道粘液分泌细胞以及一些先天/适应性免疫特征（髓过氧化物酶活性，细胞因子和在回肠和结肠中测量上皮TLR表达，肠道相关淋巴组织中免疫细胞的比例）。在HP动物的回肠中，我们观察到粘液产生细胞的增生，同时在基因和蛋白质水平上Muc2的表达增加，粘膜髓过氧化物酶活性降低，肠上皮细胞中TIr4基因表达的下调和粘膜Th的下调与肠系膜淋巴结中CD4 +淋巴细胞减少相关的细胞因子。这些变化与回肠腔中乙酸盐含量的增加相吻合。在结肠中，HP饮食摄入会导致上皮表面的杯状细胞数量减少，但结肠隐窝中的杯状细胞数量增加，同时Muc3含量增加，而11-6基因表达略有下降。我们的数据表明，HP饮食会改变结肠中杯状细胞的分布，并在回肠中增加杯状细胞的活性并降低与基础肠炎性状态有关的参数。讨论了HP饮食对肠道粘膜的有益或有害影响。 （C）2015 Elsevier Inc.保留所有权利。

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