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首页> 外文期刊>The Journal of Nutritional Biochemistry >Conjugated linoleic acid supplementation reduces peripheral blood mononuclear cell interleukin-2 production in healthy middle-aged males
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Conjugated linoleic acid supplementation reduces peripheral blood mononuclear cell interleukin-2 production in healthy middle-aged males

机译：共轭亚油酸补充减少健康中年男性外周血单个核细胞白细胞介素2的产生

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摘要

Conjugated linoleic acid (CLA) refers to geometric and positional isomers of linoleic acid. Animal studies have shown that CLA modulates the immune system and suggest that it may have a therapeutic role in inflammatory disorders. This double-blind placebo-controlled intervention trial investigated the effects of CLA supplementation on indices of immunity relating to cardiovascular disease (CVD) in a cohort of healthy middle-aged male volunteers. Subjects were randomly assigned to supplement their diet with 2.2 g 50:50 isomeric blend of cis 9, trans 11 (c9, t11)-CLA and trans 10, cis 12 (t10, c12)-CLA or placebo daily for 8 weeks. Interleukin (IL) 2, IL-10 and tumour necrosis factor (TNF) alpha were measured in the supernatant of cultured unstimulated and concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC) by ELISA. Serum IL-6 and plasma CRP were measured by ELISA and plasma fibrinogen by automated clotting assay. Gene expression was investigated by real-time RT-PCR. CLA supplementation significantly reduced Con A-stimulated PBMC IL-2 secretion (37.1%; P=.02). CLA supplementation had no significant effect on transcription of IL-2. CLA supplementation had no direct significant effects on PBMC TNFalpha or IL-10 secretion. Other inflammatory markers associated with CVD, including IL-6, CRP and fibrinogen, were not affected by CLA supplementation. This study showed that CLA supplementation reduced PBMC IL-2 secretion from Con A-stimulated PBMC but lacked effect on other markers of the human inflammatory response.
机译：共轭亚油酸（CLA）是指亚油酸的几何和位置异构体。动物研究表明，CLA可以调节免疫系统，并暗示其可能在炎症性疾病中具有治疗作用。这项双盲安慰剂对照干预试验研究了补充CLA对一群健康的中年男性志愿者的心血管疾病（CVD）免疫指数的影响。随机分配受试者饮食，每天补充2.2 g 50:50的顺式9，反式11（c9，t11）-CLA和反式10，顺式12（t10，c12）-CLA或安慰剂的异构体混合物，持续8周。通过ELISA在培养的未刺激和伴刀豆球蛋白A（Con A）刺激的外周血单个核细胞（PBMC）的上清液中测量白介素（IL）2，IL-10和肿瘤坏死因子（TNF）α。通过ELISA测定血清IL-6和血浆CRP，并通过自动凝血测定法测定血浆纤维蛋白原。通过实时RT-PCR研究基因表达。 CLA补充显着减少了Con A刺激的PBMC IL-2分泌（37.1％; P = .02）。补充CLA对IL-2的转录没有明显影响。补充CLA对PBMC TNFalpha或IL-10分泌没有直接的显着影响。与CVD相关的其他炎症标志物，包括IL-6，CRP和纤维蛋白原，不受CLA补充的影响。这项研究表明，补充CLA可以减少Con A刺激的PBMC中PBMC IL-2的分泌，但对人类炎症反应的其他标志物却没有影响。

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