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SELEX--a (r)evolutionary method to generate high-affinity nucleic acid ligands.

机译：SELEX-（r）进化方法，可产生高亲和力的核酸配体。

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摘要

SELEX stands for systematic evolution of ligands by exponential enrichment. This method, described primarily in 1990 [Ellington, A.D., Szostak, J.W., 1990. In vitro selection of RNA molecules that bind specific ligands. Nature 346, 818-822; Tuerk, C., Gold, L., 1990. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. Science 249, 505-510] aims at the development of aptamers, which are oligonucleotides (RNA or ssDNA) binding to their target with high selectivity and sensitivity because of their three-dimensional shape. Aptamers are all new ligands with a high affinity for considerably differing molecules ranging from large targets as proteins over peptides, complex molecules to drugs and organic small molecules or even metal ions. Aptamers are widely used, including medical and pharmaceutical basic research, drug development, diagnosis, and therapy. Analytical and separation tools bearing aptamers as molecular recognition and binding elements are another big field of application. Moreover, aptamers are used for the investigation of binding phenomena in proteomics. The SELEX method was modified over the years in different ways to become more efficient and less time consuming, to reach higher affinities of the aptamers selected and for automation of the process. This review is focused on the development of aptamers by use of SELEX and gives an overview about technologies, advantages, limitations, and applications of aptamers.
机译：SELEX代表通过指数富集进行配体的系统进化。该方法主要在1990年描述[Ellington，A.D.，Szostak，J.W.，1990。体外选择结合特异性配体的RNA分子。自然346，818-822; Tuerk，C.，Gold，L.，1990。通过指数富集的配体的系统进化：噬菌体T4 DNA聚合酶的RNA配体。 Science 249，505-510]旨在开发适体，由于其三维形状，它们是以高选择性和高灵敏度结合至其靶标的寡核苷酸（RNA或ssDNA）。适体是对不同分子具有高亲和力的所有新配体，范围从大的靶标，如肽上的蛋白质，复杂分子到药物以及有机小分子甚至金属离子。适体被广泛使用，包括医学和药物基础研究，药物开发，诊断和治疗。带有适体作为分子识别和结合元素的分析和分离工具是另一个重要的应用领域。而且，适体用于研究蛋白质组学中的结合现象。多年来，以各种方式对SELEX方法进行了修改，以使其变得更加高效，耗时更少，达到所选择的适体的更高亲和力以及实现过程自动化。这篇综述着重于使用SELEX开发适体，并概述了适体的技术，优势，局限性和应用。

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