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首页> 外文期刊>The Journal of Prosthetic Dentistry >Effects of provisional acrylic resins on gingival fibroblast cytokine/growth factor expression.
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Effects of provisional acrylic resins on gingival fibroblast cytokine/growth factor expression.

机译：临时丙烯酸树脂对牙龈成纤维细胞细胞因子/生长因子表达的影响。

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STATEMENT OF PROBLEM: Several studies have reported that polymerized resin materials may release agents into surrounding tissues. These agents could alter cytokine/growth factor expression. PURPOSE: The purpose of this study was to determine the effects that provisional acrylic resins have on cell toxicity and the expression of cytokines/growth factors from human gingival fibroblasts (HGFs). MATERIAL AND METHODS: The materials used in this study were chemically activated bis-acryl composite (Chem-Bis), chemically activated polyethyl methacrylate (Chem-PEMA), chemically activated polymethyl methacrylate (Chem-PMMA), and heat-activated polymethyl methacrylate (Heat-PMMA) resins. HGFs were incubated for 72 hours in the presence of eluate from each resin and in the absence of any eluate (negative control). The conditioned media were then collected and stored at -70 degrees C. Cell toxicity was determined using a lactate dehydrogenase method. Cytokine/growth factor expression was examined using cytokine antibody arrays. The experiments were repeated 3 times. The data were analyzed with 1-way ANOVA, Mann-Whitney test, and 1-sample t test (alpha=.05). RESULTS: There was no significant cell toxicity observed from the eluates. The cytokine/growth factor expression induced by Chem-Bis was significantly greater than the control for growth-regulated oncogene (GRO) (P<.001), monocyte chemoattractant protein-1 (MCP-1) (P=.031), and tumor necrosis factor-beta (TNF)-beta (P=.009). For Chem-PEMA, the cytokine/growth factor expression was significantly greater than the control for GRO-alpha (P=.022), interleukin (IL)-13 (P=.031), and TNF-alpha (P=.017). The cytokines/growth factors induced by Chem-PEMA were significantly less than the control (P=.008) and Chem-Bis for IL-8 (P=.042). The expression induced by Chem-PMMA was significantly greater than the control for IL-13 (P=.036), IL-1 alpha (P=.003), IL-2 (P=.020), and IL-5 (P=.045). Finally, Heat-PMMA induced significantly greater levels than the control for GRO (P<.001) and IL-13 (P=.008). CONCLUSIONS: This study demonstrated that the resins evaluated were nontoxic to the HGFs. There were changes in the cytokine/growth factor levels that were statistically significant, but may not be clinically significant.
机译：问题陈述：几项研究报告说，聚合树脂材料可能会将药剂释放到周围组织中。这些试剂可以改变细胞因子/生长因子的表达。目的：本研究的目的是确定临时丙烯酸树脂对人牙龈成纤维细胞（HGF）的细胞毒性和细胞因子/生长因子表达的影响。材料与方法：本研究中使用的材料为化学活化的双丙烯酸复合材料（Chem-Bis），化学活化的聚甲基丙烯酸乙酯（Chem-PEMA），化学活化的聚甲基丙烯酸甲酯（Chem-PMMA）和热活化的聚甲基丙烯酸甲酯（热-PMMA）树脂。将HGF在每种树脂的洗脱液存在下和不存在任何洗脱液的情况下孵育72小时（阴性对照）。然后收集条件培养基，并在-70℃下保存。使用乳酸脱氢酶方法测定细胞毒性。使用细胞因子抗体阵列检查细胞因子/生长因子的表达。重复实验3次。数据用1通ANOVA，Mann-Whitney检验和1样本t检验（alpha = .05）进行分析。结果：洗脱液未观察到明显的细胞毒性。 Chem-Bis诱导的细胞因子/生长因子表达显着高于生长调节癌基因（GRO）（P <.001），单核细胞趋化蛋白1（MCP-1）（P = .031）和肿瘤坏死因子-beta（TNF）-beta（P = .009）。对于Chem-PEMA，细胞因子/生长因子的表达明显高于GRO-alpha（P = .022），白介素（IL）-13（P = .031）和TNF-alpha（P = .017）的对照。 ）。 Chem-PEMA诱导的细胞因子/生长因子显着小于对照（P = .008）和Chem-Bis的IL-8（P = .042）。由Chem-PMMA诱导的表达明显高于对照组的IL-13（P = .036），IL-1 alpha（P = .003），IL-2（P = .020）和IL-5（ P = .045）。最后，Heat-PMMA诱导的水平明显高于对照组的GRO（P <.001）和IL-13（P = .008）。结论：该研究表明所评估的树脂对HGF无毒。细胞因子/生长因子水平有统计学上的显着变化，但在临床上可能没有显着变化。

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