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首页> 外文期刊>The Journal of Urology >Effect of the plant-extract osthole on the relaxation of rabbit corpus cavernosum tissue in vitro.
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Effect of the plant-extract osthole on the relaxation of rabbit corpus cavernosum tissue in vitro.

机译：植物提取物粪便对兔海绵体组织体外松弛的影响。

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摘要

PURPOSE: We investigated the cavernosal relaxant effect of osthole, a coumarin isolated from Cnidium monnier (L.) Cusson which has been long used in China as a herbal medicine to improve male sexual dysfunction. MATERIALS AND METHODS: Strips of rabbit corpus cavernosum were precontracted with phenylephrine. Corporal relaxation evoked by osthole was then determined in the absence and presence of nitric oxide synthase inhibitor (L-NAME), soluble guanylate cyclase inhibitor (ODQ), cyclooxygenase inhibitor (indomethacin), tetradotoxin, and after endothelium deprivation. RESULTS: Corpus cavernosal strips showed relaxation in response to osthole (0.1 approximately 30 microM) in a dose-dependent manner. These effects were reduced partially but significantly by pretreatment with L-NAME, ODQ and by endothelial disruption. However, they were not affected by indomethacin and tetradotoxin treatment. Osthole pretreatment (from 1 to 30 microM) enhanced the sodium nitroprusside (0.3 microM)-induced relaxation of corpus cavernosum in a dose-dependent manner to a maximum of 3 times the pretreatment level at 30 microM osthole. However, this effect was abolished in the presence of zaprinast. Additionally, a higher concentration of osthole (30 microM) also enhanced forskolin-induced relaxation. CONCLUSION: The data suggested that osthole possesses a relaxant effect on rabbit corpus cavernosal tissues which is attributable to the release of NO from sinusoidal endothelium and to the potentiation of the cGMP and/or cAMP signal mediating relaxation of cavernosal smooth muscle by inhibiting phosphodiesterase.
机译：目的：我们研究了蛇床子香豆素的海绵体松弛作用，蛇床子香豆素是从Cndium monnier（L.）Cusson分离得到的香豆素，在中国长期以来一直用作改善男性性功能障碍的草药。材料与方法：将兔海绵体条与去氧肾上腺素预收缩。然后在缺乏和存在一氧化氮合酶抑制剂（L-NAME），可溶性鸟苷酸环化酶抑制剂（ODQ），环加氧酶抑制剂（吲哚美辛），四毒菌素和内皮剥夺后，测定由粪便引起的体质松弛。结果：海绵体海绵体条显示出对骨hole（0.1约30 microM）的反应，呈剂量依赖性。通过用L-NAME，ODQ预处理和内皮破坏，这些作用会部分但显着降低。但是，它们不受消炎痛和四毒菌素治疗的影响。 Osthole预处理（从1到30 microM）以剂量依赖的方式增强了硝普钠（0.3 microM）诱导的海绵体松弛，最大剂量为30 microM osthole时的预处理水平的3倍。但是，在存在扎普利斯特的情况下，这种作用被消除。此外，较高的蛇床子素浓度（30 microM）也增强了毛喉素诱导的松弛。结论：数据表明，蛇床子素对兔海绵体组织具有松弛作用，这归因于从窦状内皮中释放的NO以及cGMP的增强和/或cAMP信号通过抑制磷酸二酯酶介导海绵体平滑肌的松弛。

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