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首页> 外文期刊>The journal of sexual medicine >Cervix stimulation evokes predominantly subthreshold synaptic responses in mouse thoracolumbar and lumbosacral superficial dorsal horn neurons.
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Cervix stimulation evokes predominantly subthreshold synaptic responses in mouse thoracolumbar and lumbosacral superficial dorsal horn neurons.

机译：子宫颈刺激在小鼠胸腰和腰s浅背角神经元中主要引起阈下的突触反应。

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INTRODUCTION: Sensory input from the female reproductive tract (FRT) plays a pivotal role in coordinating reproductive reflexes. Additionally, a number of disorders, especially chronic pelvic pain, may be due to disturbances in sensory processing of signals from the FRT. AIMS: Our aim was to record synaptic responses in neurons from lumbar and sacral spinal cord segments during mechanical stimulation of the cervix. METHODS: We developed an in vivo preparation of the mouse spinal cord to record synaptic potentials from superficial dorsal horn (SDH) neurons under whole-cell patch clamp recording conditions. MAIN OUTCOME MEASURES: We analyzed the strength and distribution of excitatory postsynaptic potentials in SDH neurons evoked during mechanical stimulation of the cervix and cutaneous sites. RESULTS: Resting membrane potential and neuronal input resistance was similar in thoracolumbar (TL, T13-L3) and lumbosacral (LS, L6-S2) segments. We elicited activity in 6/21 TL neurons and 15/39 LS neurons using mechanical stimulation of the cervix with a blunt probe. The majority of these neurons responded to cervix stimulation with bursts of subthreshold excitatory postsynaptic potentials (4/6 and 12/15 TL and LS neurons, respectively). The remainder responded with sufficient magnitude to generate action potentials (2/6 and 3/15 TL and LS neurons). Cutaneous synaptic inputs were also elicited in 11/21 TL neurons following stimulation of the flank/leg, 19/39 LS neurons by stimulation of the tail, and three LS neurons by perineal stimulation. Some neurons received convergent synaptic inputs from the cervix and cutaneous sites (4/6 TL and 4/15 LS). CONCLUSION: These data demonstrate that spinal projections of cervix afferents are widely dispersed in the SDH and considerable convergence exists between neurons innervating the cervix and cutaneous structures. Our results indicate that much of the synaptic activity evoked in SDH neurons following cervix stimulation is subthreshold.
机译：简介：女性生殖道（FRT）的感觉输入在协调生殖反射中起关键作用。另外，许多疾病，尤其是慢性骨盆痛，可能是由于FRT信号的感觉处理受到干扰。目的：我们的目的是在机械刺激子宫颈期间记录来自腰椎和spin脊髓节段神经元的突触反应。方法：我们开发了一种小鼠脊髓的体内制剂，以在全细胞膜片钳记录条件下记录来自浅背角（SDH）神经元的突触电位。主要观察指标：我们分析了在机械刺激子宫颈和皮肤部位期间诱发的SDH神经元中兴奋性突触后电位的强度和分布。结果：在胸腰段（TL，T13-L3）和腰s段（LS，L6-S2）中，静息膜电位和神经元输入阻力相似。我们使用钝探针对子宫颈进行机械刺激，在6/21 TL神经元和15/39 LS神经元中引起活性。这些神经元中的大多数对子宫颈刺激有阈下兴奋性突触后电位爆发（分别为4/6和12/15 TL和LS神经元）。其余的反应幅度足以产生动作电位（2/6和3/15 TL和LS神经元）。在刺激侧翼/腿后，还会在11/21 TL神经元中引发皮肤突触输入，通过刺激尾巴会刺激19/39 LS神经元，会阴刺激会刺激三个LS神经元。一些神经元从子宫颈和皮肤部位（4/6 TL和4/15 LS）接受会聚的突触输入。结论：这些数据表明子宫颈传入神经的脊柱投射广泛分布在SDH中，支配子宫颈的神经元与皮肤结构之间存在相当大的收敛性。我们的结果表明，子宫颈刺激后在SDH神经元中诱发的许多突触活动处于阈值以下。

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