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首页> 外文期刊>The journal of sexual medicine >Vardenafil and resveratrol synergistically enhance the nitric oxide/cyclic guanosine monophosphate pathway in corpus cavernosal smooth muscle cells and its therapeutic potential for erectile dysfunction in the streptozotocin-induced diabetic rat: preliminary findings.
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Vardenafil and resveratrol synergistically enhance the nitric oxide/cyclic guanosine monophosphate pathway in corpus cavernosal smooth muscle cells and its therapeutic potential for erectile dysfunction in the streptozotocin-induced diabetic rat: preliminary findings.

机译：伐地那非和白藜芦醇协同增强链脲佐菌素诱发的糖尿病大鼠海绵体平滑肌细胞中一氧化氮/环状鸟苷单磷酸途径及其对勃起功能障碍的治疗潜力：初步发现。

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INTRODUCTION: Phosphodiesterase type 5 (PDE5) inhibitors are very effective agents for erectile dysfunction; however, specific patient populations are hard to treat. The efficacy of PDE5 inhibitors is limited because a minimum amount of nitric oxide (NO) is necessary. Resveratrol, a plant polyphenol, is reported to activate endothelial NO synthase (eNOS) through activation of sirtuin 1. We previously reported that human corpus cavernosal smooth muscle cells (CCSMCs) express eNOS and synthesize cyclic guanosine monophosphate (cGMP) via the NO/cGMP pathway. AIM: To investigate the ability of resveratrol and/or vardenafil to increase cGMP in an in vitro model using CCSMCs and to improve erectile function in an in vivo rat model of streptozotocin (STZ)-induced diabetes. METHODS: CCSMCs were treated with resveratrol and/or vardenafil. Twenty male Sprague-Dawley rats were randomly divided into five groups (N = 4 in each group): age-matched controls, diabetic controls, and diabetic rats treated with resveratrol, vardenafil, or both in combination for the last 4 weeks of an 8-week period of diabetes induction. MAIN OUTCOME MEASURES: Intracellular cGMP measurement, intracovernous pressure (ICP)/mean arterial pressure (MAP) ratio, and smooth muscle/collagen ratio. RESULTS: Intracellular cGMP level was elevated by resveratrol treatment in CCSMCs. The combination treatment of resveratrol and vardenafil had a synergistic effect. Diabetic rats showed impairment of erectile function. Treatment with either resveratrol or vardenafil improved ICP/MAP ratio, and combination therapy with resveratrol and vardenafil had a synergistic effect in improvement of ICP/MAP. CONCLUSIONS: Treatment with either resveratrol or vardenafil elevated cGMP level in CCSMCs and improved erectile function in STZ-induced diabetic rats. Furthermore, a synergistic effect was observed in vitro and in vivo. Resveratrol or combination therapy of resveratrol and vardenafil can improve erectile function in which NO release is impaired, although further study is needed to confirm the results.
机译：简介：磷酸二酯酶5型（PDE5）抑制剂是治疗勃起功能障碍的非常有效的药物。但是，特定的患者人群很难治疗。 PDE5抑制剂的功效受到限制，因为必须使用最少量的一氧化氮（NO）。据报道，白藜芦醇是一种植物多酚，可通过激活sirtuin 1来激活内皮NO合酶（eNOS）。我们先前曾报道人类海绵体平滑肌细胞（CCSMC）表达eNOS并通过NO / cGMP合成环状鸟苷单磷酸（cGMP）。途径。目的：研究在使用CCSMC的体外模型中白藜芦醇和/或伐地那非提高cGMP的能力以及在链脲佐菌素（STZ）诱导的糖尿病体内模型中改善勃起功能的能力。方法：CCSMCs用白藜芦醇和/或伐地那非治疗。将20只雄性Sprague-Dawley大鼠随机分为五组（每组N = 4）：年龄匹配的对照组，糖尿病对照组和接受白藜芦醇，伐地那非或两者合用的糖尿病大鼠，共8次，共8周。周糖尿病诱导期。主要观察指标：细胞内cGMP测量，表皮内压力（ICP）/平均动脉压（MAP）比和平滑肌/胶原蛋白比。结果：CCSMCs中白藜芦醇处理可提高细胞内cGMP水平。白藜芦醇和伐地那非的联合治疗具有协同作用。糖尿病大鼠显示勃起功能受损。白藜芦醇或伐地那非治疗可改善ICP / MAP比，白藜芦醇和伐地那非联合治疗可改善ICP / MAP。结论：白藜芦醇或伐地那非治疗可提高CCSMCs的cGMP水平，并改善STZ诱导的糖尿病大鼠的勃起功能。此外，在体外和体内观察到协同作用。白藜芦醇或白藜芦醇与伐地那非的联合治疗可改善勃起功能，其中NO释放受到损害，尽管还需要进一步的研究来证实结果。

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