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首页> 外文期刊>The Korean Journal of Genetics >Apoptosis and DNA repair of Chinese hamster ovary cells exposed to DNA damaging agents
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Apoptosis and DNA repair of Chinese hamster ovary cells exposed to DNA damaging agents

机译：暴露于DNA损伤剂的中国仓鼠卵巢细胞的凋亡和DNA修复

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摘要

The present study has been performed to elucidate apoptosis and DNA repair to DNA damaging agents in Chinese hamster ovary (CHO) cells. Four assays were employed in this study; DNA fragmentation by agarose gel electrophoresis, quantitative assay offragmented DNA, morphological assessment of apoptotic cells and expression of heat shock protein (Hsp 70) visualized by western blot analysis. DNA fragmentation in cells treated with ultraviolet radiation (UV), heat or actinomycin D (AMD) was induced attwelve, six or nine hour, respectively. The level of Hsp 70 in cells pretreated with low dose of UV or heat and subsequently treated with high dose of UV or heat was higher than that treated with high dose of UV or heat. The level of Hsp 70 in cells preheated for five minutes at 43 deg C and subsequently treated with 5 #mu#g/ml AMD was higher than that only treated with 5 #mu#g/ml AMD. DNA fragmentation was delayed in cells pretreated with low dose of UV or heat. These results suggest that the incrementof Hsp 70 relates with a delay of DNA fragmentation associated with apoptosis and DNA repair such as adaptive response.
机译：进行本研究以阐明中国仓鼠卵巢（CHO）细胞的凋亡和DNA修复剂对DNA损伤的作用。在这项研究中采用了四种检测方法。通过琼脂糖凝胶电泳对DNA进行片段化，对碎片化的DNA进行定量测定，对凋亡细胞进行形态学评估，并通过Western印迹分析显示热休克蛋白（Hsp 70）的表达。分别在第12、6或9个小时诱导了用紫外线（UV），热或放线菌素D（AMD）处理的细胞中的DNA断裂。用低剂量的紫外线或热预处理，然后用高剂量的紫外线或热处理的细胞中Hsp 70的水平高于用高剂量的紫外线或热处理的细胞。在43摄氏度下预热5分钟并随后用5＃mu＃g / ml AMD处理的细胞中Hsp 70的水平高于仅用5＃mu＃g / ml AMD处理的细胞中的Hsp 70的水平。在用低剂量的紫外线或热预处理的细胞中，DNA断裂被延迟。这些结果表明，Hsp 70的增加与与细胞凋亡和DNA修复（如适应性反应）相关的DNA片段化的延迟有关。

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