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首页> 外文期刊>The Korean Journal of Genetics >Genetic Differences and Variations in Slipper Lobster (Ibacus ciliatus) and Deep Sea Lobster (Puerulus sewelli) Determined by RAPD Analysis
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Genetic Differences and Variations in Slipper Lobster (Ibacus ciliatus) and Deep Sea Lobster (Puerulus sewelli) Determined by RAPD Analysis

机译：RAPD分析确定拖鞋龙虾（Ibacus ciliatus）和深海龙虾（Puerulus sewelli）的遗传差异和变异

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摘要

We have isolated the genomic DNA for random amplified polymorphic DNA-polymerase chain reaction RAPD-PCR from two geographic lobster species of Korea and the Indian Ocean. The complexity of the banding patterns varied dramatically between the primers and locales from the two locations. We used eight decamer and 20-mer primers to generate a total of 510 fragments in a species of Korean slipper lobster Ibacus ciliatus, and 526 in a species of Indian Ocean deep sea lobster Puerulus sewelli, with DNA fragment sizes ranging from 100 bp to 2,000 bp: 106 specific fragments 20.8% in the Korean lobster species and 136 25.9% in the Indian Ocean species. 242 intra-species-specific fragments, with an average of 30.3 per primer, were observed in the Korean species. 187 fragments, with an average of 23.4 per primer, were identified in the Indian Ocean species. Especially, 132 intra-species-common fragments, with an average of 16.5 per primer, were observed in the Korean species. The OPA-18 decamer primer also generated inter-species-common DNA fragments, with sizes of approximately 150 bp, 200 bp, and 300 bp, respectively, in both the Korean and the Indian Ocean lobster species. The average bandsharing BS value of individuals within the Indian Oceanspecies 0.742 + 0.009 was much higher than in the Korean lobster species 0.710 0.009. The dendrogram obtained with the eight primers indicates two genetic clusters, designated cluster 1 SLIPPER 01 SLIPPER 11, and cluster 2 DEEPSEA 12 DEEPSEA22. Ultimately, the longest genetic distance displaying significant molecular differences was determined to exist between the Korean and the Indian Ocean lobster species genetic distance = 0.612.
机译：我们已经从韩国和印度洋的两个地理龙虾物种中分离出用于随机扩增的多态性DNA-聚合酶链反应RAPD-PCR的基因组DNA。带状图谱的复杂性在两个位置的引物和位置之间变化很大。我们使用了八个decamer和20-mer引物，在朝鲜拖鞋龙虾Ibacus ciliatus的物种中产生了510个片段，在印度洋深海龙虾Puerulus sewelli的物种中产生了526个片段，DNA片段大小在100 bp至2,000之间bp：在韩国龙虾物种中有106个特定片段，占20.8％，在印度洋物种中，有136个25.9％。在朝鲜族物种中观察到242种物种内特异性片段，每个引物平均30.3。在印度洋物种中鉴定出187个片段，平均每个引物23.4个。特别是，在朝鲜族物种中观察到132种物种内常见片段，平均每个引物16.5。 OPA-18 decamer引物还在韩国和印度洋龙虾物种中也产生了物种间常见的DNA片段，大小分别约为150 bp，200 bp和300 bp。印度洋物种内个体的平均带状共享BS值0.742 + 0.009远高于韩国龙虾物种0.710 0.009。用八种引物获得的树状图显示两个遗传簇，分别称为簇1 SLIPPER 01 SLIPPER 11和簇2 DEEPSEA 12 DEEPSEA22。最终，确定在韩国和印度洋龙虾物种遗传距离= 0.612之间存在着表现出明显分子差异的最长遗传距离。

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