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首页> 外文期刊>The Korean Journal of Genetics >Nucleotide sequence complexity of apolipoprotein C-II 3 ' UTR in the rainbow trout (Oncorhynchus mykiss): Alternatively polyadenylated transcripts and phylogenetic association with the FokI SINE element in Salmonids
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Nucleotide sequence complexity of apolipoprotein C-II 3 ' UTR in the rainbow trout (Oncorhynchus mykiss): Alternatively polyadenylated transcripts and phylogenetic association with the FokI SINE element in Salmonids

机译：虹鳟（Oncorhynchus mykiss）中载脂蛋白C-II 3'UTR的核苷酸序列复杂性：鲑鱼中的聚腺苷酸化转录物以及与FokI SINE元素的系统发育关联

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摘要

6 different polyadenylated variants (apo C-II v1 to v6) were found in rainbow trout apo C-H gene from the rainbow trout EST analysis. Apo C-II v1 and v2 did not contain the putatitive transcriptional binding site of HNF-3 beta due to the incomplete sequence of 3' UTR of apo C-II. Apo C-II v2 to v6 all contain an Fokl short interspersed element (SINE), located in the 3' untraslated region (UTR). The Fokl element was associated with various genes only from aquatic speices. Fokl specific PCR products were produced, which suggests the interspersed nature of distribution of the Fokl element. PCR using primers from the Fokl SINE and the apo C-II 3' UTR region indicates that the apo C-II cDNA is structurally conserved among Salmonids and that these primers may be useful for Salmonid species genotyping. Variation in the sequence and copy number of the Fokl SINE in the Salmonid species has allowed their use as a marker of genetic variation.
机译：根据虹鳟EST分析，在虹鳟apo C-H基因中发现了6种不同的聚腺苷酸化变体（apo C-II v1至v6）。由于载脂蛋白C-II 3'UTR的序列不完整，载脂蛋白C-II v1和v2不包含HNF-3 beta的推定转录结合位点。 Apo C-II v2至v6均包含位于3'非翻译区（UTR）中的Fokl短散布元素（SINE）。 Fokl元件仅与水生物种中的各种基因相关。产生了Fokl特异性PCR产物，这暗示了Fokl元件分布的散布性质。使用来自Fokl SINE和载脂蛋白C-II 3'UTR区的引物进行PCR表明，载脂蛋白C-II cDNA在鲑鱼中结构保守，这些引物可用于鲑鱼种的基因分型。鲑鱼物种中Fokl SINE的序列和拷贝数的变化已使其可以用作遗传变异的标记。

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