...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of toxicological sciences >Endocrine disrupting effects of low dose 17 beta-estradiol (E2) on the Japanese quail (Coturnix japonica) were detected by modified one-generation reproduction study.
【24h】

Endocrine disrupting effects of low dose 17 beta-estradiol (E2) on the Japanese quail (Coturnix japonica) were detected by modified one-generation reproduction study.

机译：通过改进的一代生殖研究，研究了低剂量17β-雌二醇（E2）对日本鹌鹑（Coturnix japonica）的内分泌干扰作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Previously, we investigated endocrine disrupting effects of 17 beta-estradiol (E(2)) on Japanese quail (Coturnix japonica) in the avian reproduction test according to the testing guidelines, in which new endpoints such as blood vitellogenin (VTG) concentration in parent quails and pathology of F(1) chicks were added, and consequently these additional endpoints suggested to be sensitive markers for detecting any impacts of endocrine disrupting effects (Shibuya et al., 2005b). In the present study, to investigate low dose effects of estrogenic endocrine disrupting chemicals in birds, the avian reproduction study of E(2) at low dose levels was conducted using Japanese quail with additional endpoints such as observations of F(1) chicks until 10 weeks of age, histopathology of F(1) chicks at 14 days and 10 weeks of age and blood VTG concentration in parent quails. Sixteen pairs of 10-week-old quails were fed a low phytoestrogen diet containing E(2) at 0 (control), 0.3, 3, and 30 ppm for 6 weeks, and parent quails, eggs and offspring were examined. F(1) chicks were maintained up to 14 days or 10 weeks of age. Serum E(2) and VTG concentrations in males of the E(2) 3- and 30-ppm groups and in females of the E(2) 30-ppm groups were significantly elevated. In the E(2) 30-ppm group, two parent females died, and toxic changes such as suppression of body weight gain, decrease in food consumption and atrophic and degenerative changes of the reproductive organs were observed in parent quails. In the same group, the number of eggs laid and the fertility rate of eggs were significantly decreased. In addition, the viability of F(1) chicks in the E(2) 30-ppm group were significantly decreased at 10 weeks of age. On the other hand, no abnormalities described above were observed in any parent quails, eggs and F(1) chicks in the E(2) 3- and 0.3-ppm groups, although the fertility rates of eggs in both groups were decreased and the body weight gain of F(1) females in the E(2) 3-ppm group was significantly suppressed. In the histopathological examination of F(1) chicks maintained up to 10 weeks of age, persistent right oviduct and atrophy of the oviduct gland were observed in females of E(2)-treatment groups with significantly high incidences. Moreover, cystic dilatation and tubular degeneration of the seminiferous tubules and atrophy of the cloacal gland were also observed in males of the E(2)-treatment groups. Thus, the dietary treatment of low dose E(2) (even 0.3 ppm) to parent quails resulted in decreased viability and induction of abnormalities in the oviduct, testis and cloacal gland in F(1) chicks maintained up to 10 weeks of age. These results suggest that additional endpoints such as observations of F(1) chicks until 10 weeks of age, histopathology of F(1) chicks at 14 days and 10 weeks of age and blood VTG concentration in parent quails would be useful and sensitive endpoints for evaluating estrogenic endocrine disrupting effects in the avian reproduction study.
机译：以前，我们根据测试指南调查了17β-雌二醇（E（2））对日本鹌鹑（Coturnix japonica）的内分泌干扰作用，该试验遵循新的终点，例如父母体内血液卵黄蛋白原（VTG）的浓​​度添加了鹌鹑和F（1）小鸡的病理学，因此，这些额外的终点被认为是检测内分泌干扰作用的任何敏感标记（Shibuya等，2005b）。在本研究中，为了研究鸟类中雌激素内分泌干扰物的低剂量效应，使用日本鹌鹑进行了低剂量水平的E（2）禽类繁殖研究，并附加了终点，例如观察到F（1）小鸡，直到10只。周龄，14天和10周龄F（1）雏鸡的组织病理学以及父母鹌鹑的血液VTG浓度。向16对10周龄的鹌鹑喂饲低E（2）含量为0（对照），0.3、3和30 ppm的低植物雌激素饲料，持续6周，并检查父母鹌鹑，卵和后代。 F（1）小鸡维持到14天或10周龄。 E（2）3-和30-ppm组的男性和E（2）30-ppm组的女性的血清E（2）和VTG浓度显着升高。在E（2）30 ppm组中，有两名雌性母鸡死亡，并且在雌性鹌鹑中观察到毒性变化，例如抑制体重增加，食物消耗减少以及生殖器官萎缩和退化性变化。在同一组中，产卵数和卵的受精率明显降低。此外，E（2）30 ppm组中的F（1）小鸡的生存力在10周龄时显着降低。另一方面，在E（2）3-和0.3-ppm组中，任何亲代鹌鹑，卵和F（1）小鸡均未观察到上述异常，尽管两组中卵的受精率均降低且E（2）3-ppm组中F（1）女性的体重增加受到显着抑制。在F（1）小鸡维持到10周龄的组织病理学检查中，在E（2）-治疗组的女性中观察到持续的右输卵管和输卵管腺体萎缩，发病率极高。此外，在E（2）-治疗组的男性中也观察到了曲细精管的囊性扩张和肾小管变性以及泄殖腔的萎缩。因此，对父母鹌鹑低剂量E（2）（甚至0.3 ppm）的饮食治疗导致存活率降低，并导致F（1）雏鸡的输卵管，睾丸和泄殖腔异常，直至10周龄。这些结果表明，其他终点，例如直到10周龄的F（1）雏鸡的观察，14天和10周龄的F（1）雏鸡的组织病理学以及父母鹌鹑中的血液VTG浓度对于有用的终点也很有用在禽繁殖研究中评估雌激素内分泌干扰作用。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号