• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of toxicological sciences >Differences in micronucleus induction in peripheral blood reticulocytes of mice exposed to N-ethyl-N-nitrosourea at light and dark dosing times.
【24h】

Differences in micronucleus induction in peripheral blood reticulocytes of mice exposed to N-ethyl-N-nitrosourea at light and dark dosing times.

机译：在明和暗给药时间暴露于N-乙基-N-亚硝基脲的小鼠外周血网织红细胞中微核诱导的差异。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mammals, including human beings, have a circadian clock system to regulate behavioral and physiological processes. In this study, we investigated the effect of dosing time on micronucleus induction in the bone marrow by evaluating the frequencies of micronucleated peripheral reticulocytes (MNRETs) in mice exposed to N-ethyl-N-nitrosourea (ENU) to assess any difference in genotoxic sensitivity to chemicals between light and dark periods (inactive phase for rodents and active phase for rodents). Male C3H/He mice were treated intraperitoneally with ENU (12.5 or 25 mg/kg body weight) at zeitgeber time (ZT) 3 in the light period or ZT15 in the dark period, and then the time courses of the frequencies of the MNRETs were determined. The frequencies of the MNRETs induced by ENU increased time-dependently and peaked at 48 hr after treatment for ZT3 and ZT15, and were obviously higher in the ZT15 treatment group than the ZT3 treatment group. The MNRETs were measured at 48 hr after treatment with ENU (25 mg/kg body weight) at various dosing times (ZT0, 3, 6, 12, 15 and 18). The frequencies of the MNRETs in mice treated at ZT0, 15 and 18 were significantly higher than those in mice treated at ZT3, 6 and 12. These results suggest that genotoxic sensitivity to chemicals in mouse bone marrow is different between light and dark periods maybe due to different biological responses (detoxification, cell cycle, DNA repair, etc.) related to circadian rhythms.
机译：包括人类在内的哺乳动物都有一个昼夜节律系统来调节行为和生理过程。在这项研究中，我们通过评估暴露于N-乙基-N-亚硝基脲（ENU）的小鼠中微核外周网织细胞（MNRETs）的频率来评估给药时间对骨髓中微核诱导的影响，以评估遗传毒性敏感性的任何差异在黑暗和黑暗时期（啮齿类动物处于非活性相，啮齿类动物处于活性相）之间的化学物质。雄性C3H / He小鼠在光亮时期的Zeitgeber时间（ZT）3或黑暗时期的ZT15接受ENU（12.5或25 mg / kg体重）腹膜内治疗，然后MNRET的频率随时间变化决心。 ZT3和ZT15治疗后，由ENU诱导的MNRETs的频率随时间增加，并在48小时达到峰值，ZT15治疗组明显高于ZT3治疗组。在不同剂量（ZT0、3、6、12、15和18）用ENU（25 mg / kg体重）治疗后48小时测量MNRET。在ZT0、15和18处理的小鼠中MNRET的频率显着高于在ZT3、6和12处理的小鼠。这些结果表明，在明亮和黑暗时期，小鼠骨髓对化学物质的遗传毒性敏感性不同。对与昼夜节律有关的不同生物学反应（排毒，细胞周期，DNA修复等）的反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号