• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of toxicological sciences >Effect of talaporfin sodium-mediated photodynamic therapy on cell death modalities in human glioblastoma T98G cells
【24h】

Effect of talaporfin sodium-mediated photodynamic therapy on cell death modalities in human glioblastoma T98G cells

机译：他拉泊芬钠介导的光动力疗法对人胶质母细胞瘤T98G细胞死亡方式的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

While photodynamic therapy (PDT) is an effective treatment for glioma, induction of apoptotic cell death of glioma cells is important for ensuring efficacy and safety of PDT treatment in glioma patients, as necrotic cell death can induce late appearance of obstacles in treatment. Here, we investigated the relationship between type of cell death and PDT treatment conditions involved in laser and photosensitizer dosage in human glioblastoma T98G cells. Photosensitizer talaporfin sodium-mediated PDT (NPe6-PDT) treatment induced laser and NPe6 dose-dependent cell death in T98G cells, whereas almost all cells pretreated with NPe6 at >= 30 11 mu g/mL were killed by laser irradiation, regardless of laser dose. Morphological analysis showed that combination of high doses of NPe6 and laser irradiation changes the dominant cell death process from apoptosis to necrosis. Biochemical analysis (detection of caspase-3 activity and staining of cell surface-exposed phosphatidylserine) also showed that increasing laser dose changes the type of cell death from apoptotic to necrotic cell death after high-dose treatment with NPe6. Lactate dehydrogenase leakage assay demonstrated that a laser dose of 5 J/cm(2) induced less leakage than 30 J/cm(2). Our results suggested that type of glioma cell death in NPe6-PDT changed with fluctuations in laser and NPe6 dose, and that combination of 30m mu g/mL NPe6 with 5 J/cm(2) laser is the best treatment condition for inducing an increase in apoptotic cells while keeping rate of necrotic cell death low in this in vitro study.
机译：尽管光动力疗法（PDT）是治疗神经胶质瘤的有效方法，但诱导神经胶质瘤细胞凋亡性细胞死亡对于确保PDT治疗胶质瘤患者的疗效和安全性很重要，因为坏死性细胞死亡可导致治疗后期出现障碍。在这里，我们调查了人类胶质母细胞瘤T98G细胞中激光和光敏剂剂量所涉及的细胞死亡类型与PDT治疗条件之间的关系。用光敏剂他拉泊芬钠介导的PDT（NPe6-PDT）处理可在T98G细胞中诱导激光和NPe6剂量依赖性细胞死亡，而几乎所有用NPe6≥30 11μg / mL预处理的细胞均被激光照射杀死，而与激光无关剂量。形态学分析表明，高剂量的NPe6和激光照射相结合会改变从凋亡到坏死的主要细胞死亡过程。生化分析（检测caspase-3活性和对细胞表面暴露的磷脂酰丝氨酸染色）还显示，增加剂量的激光将NPe6大剂量处理后的细胞死亡类型从凋亡性转变为坏死性细胞死亡。乳酸脱氢酶泄漏测定表明，激光剂量为5 J / cm（2）引起的泄漏少于30 J / cm（2）。我们的研究结果表明，NPe6-PDT中神经胶质瘤细胞死亡的类型随激光和NPe6剂量的变化而变化，并且30mμg / mL NPe6与5 J / cm（2）激光的组合是诱导增加的最佳治疗条件在这项体外研究中，它可以使凋亡细胞降低坏死细胞死亡率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号