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首页> 外文期刊>The New Phytologist >Competition between plant and bacterial cells at the microscale regulates the dynamics of nitrogen acquisition in wheat (Triticum aestivum)
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Competition between plant and bacterial cells at the microscale regulates the dynamics of nitrogen acquisition in wheat (Triticum aestivum)

机译：植物和细菌细胞之间的竞争在微观上调节了小麦（Triticum aestivum）氮素吸收的动态。

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The ability of plants to compete effectively for nitrogen (N) resources is critical to plant survival. However, controversy surrounds the importance of organic and inorganic sources of N in plant nutrition because of our poor ability to visualize andunderstand processes happening at the root-microbial-soil interface. Using high-resolution nano-scale secondary ion mass spectrometry stable isotope imaging (NanoSIMS-SII), we quantified the fate of ~(15)N over both space and time within the rhizosphere.We pulse-labelled the soil surrounding wheat (Triticum aestivum) roots with either or ~(15)N-glutamate and traced the movement of ~(15)N over 24 h. Imaging revealed that glutamate was rapidly depleted from the rhizosphere and that most ~(15)N was captured by rhizobacteria, leading to very high ~(15)N microbial enrichment. After microbial capture, approximately half of the ~(15)N-glutamate was rapidly mineralized, leading to the excretion of , which became available for plant capture. Roots proved to be poor competitors for ~(15)N-glutamate and took up N mainly as . Spatial mapping of ~(15)N revealed differential patterns of ~(15)N uptake within bacteria and the rapid uptake and redistribution of ~(15)N within roots. In conclusion, we demonstrate therapid cycling and transformation of N at the soil-root interface and that wheat capture of organic N is low in comparison to inorganic N under the conditions tested.
机译：植物有效竞争氮（N）资源的能力对于植物生存至关重要。然而，由于我们对根与微生物-土壤界面处发生的过程的可视化和理解能力差，围绕植物营养中有机和无机氮源的重要性一直存在争议。我们使用高分辨率纳米级二次离子质谱稳定同位素成像（NanoSIMS-SII）定量分析了根际内〜（15）N在空间和时间上的命运，并对小麦周围的土壤进行了脉冲标记（Triticum aestivum） ）或〜（15）N谷氨酸根生长，并追踪〜（15）N在24小时内的运动。成像显示谷氨酸迅速从根际中耗尽，并且大多数〜（15）N被根际细菌捕获，导致很高的〜（15）N微生物富集。微生物捕获后，大约一半的〜（15）N-谷氨酸迅速矿化，导致的排泄，可用于植物捕获。根被证明是〜（15）N-谷氨酸的弱竞争者，主要以N吸收N。 〜（15）N的空间作图显示细菌中〜（15）N吸收的差异模式以及根中〜（15）N的快速吸收和重新分布。总之，我们证明了在土壤-根界面上氮的有效循环和转化，并且在测试条件下，小麦对有机氮的捕获量比无机氮低。

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