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首页> 外文期刊>The New Phytologist >Approximating subcellular organisation of carbohydrate metabolism during cold acclimation in different natural accessions of Arabidopsis thaliana
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Approximating subcellular organisation of carbohydrate metabolism during cold acclimation in different natural accessions of Arabidopsis thaliana

机译：拟南芥不同自然部位冷驯化过程中碳水化合物代谢的亚细胞组织

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摘要

Accessions of Arabidopsis thaliana originating from climatically different habitats show different levels of cold acclimation when exposed to low temperatures. The central carbohydrate metabolism plays a crucial role during this acclimation. Subcellular distribution of carbohydrates over the compartments cytosol, vacuole and plastids, and putative interactions of the compartments, are analyzed in three differentially cold-tolerant accessions of Arabidopsis thaliana, originating from the Iberian Peninsula (C24), Russia (Rschew) and Scandinavia (Tenela), respectively. Subcellular carbohydrate concentrations were determined by applying the nonaqueous fractionation technique. Mathematical modeling and steady-state simulation was used to analyse the metabolic homeostasis during cold exposure. In all accessions, the initial response to cold exposure was a significant increase of plastidial and cytosolic sucrose concentrations. Raffinose accumulated in all cellular compartments of cold-tolerant accessionswith a delay of 3 d, indicating that raffinose accumulation is a long-term component of cold acclimation. Minimal rates of metabolite transport permitting steady-state simulations of metabolite concentrations correlated with cold tolerance, indicating an important role of subcellular re-distribution of metabolites during cold acclimation. A highly regulated interplay of enzymatic reactions and intracellular transport processes appears to be a prerequisite for maintaining carbohydrate homeostasis during cold exposure and allowing cold acclimation in Arabidopsis thaliana.
机译：源自气候不同栖息地的拟南芥种在暴露于低温时表现出不同水平的冷驯化。在这种适应过程中，中央碳水化合物的代谢起着至关重要的作用。在来自伊比利亚半岛（C24），俄罗斯（Rschew）和斯堪的纳维亚（Tenela）的拟南芥的三种不同耐寒性种质中分析了碳水化合物在隔室中的亚细胞分布胞质，液泡和质体以及隔室的假定相互作用）， 分别。通过应用非水分离技术确定亚细胞碳水化合物的浓度。数学建模和稳态模拟用于分析冷暴露期间的代谢稳态。在所有种质中，对冷暴露的最初反应是质体和胞质蔗糖浓度的显着增加。耐高温种质的所有细胞区中的棉子糖累积滞后3 d，表明棉子糖累积是冷驯化的长期组成部分。最低限度的代谢物运输速率允许对代谢物浓度进行稳态模拟，并与耐寒性相关，这表明在冷驯化过程中代谢物的亚细胞重新分布具有重要作用。酶反应和细胞内运输过程之间高度受控的相互作用似乎是维持冷暴露过程中糖类稳态并允许拟南芥进行冷驯化的先决条件。

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