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首页> 外文期刊>The New Phytologist >StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst
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StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst

机译：StCDPK5赋予对晚疫病病原菌的抗性，但通过活性氧的爆发增加了马铃薯对早疫病病原菌的敏感性

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摘要

Potato (Soianum tuberosum) calcium-dependent protein kinase (StCDPKS) has been shown to phosphorylate the N-terminal region of plasma membrane RBOH (respiratory burst oxidase homolog) proteins, and participate in StRBOHB-mediated reactive oxygen species (ROS) burst. The constitutively active form, StCDPKSVK, provides a useful tool for gain-of-function analysis of RBOH in defense responses. StCDPK5- and StCDPK5VK-green fluorescent protein fusion proteins were predominantly targeted to the plasma membrane, and conditional expression of StCDPKSVK activated StRBOHA-D. The interaction was confirmed by bimolecular fluorescence complementation assay. We generated transgenic potato plants containing StCDPK5VK under the control of a pathogen-inducible promoter to investigate the role of ROS burst on defense responses to blight pathogens. Virulent isolates of the late blight pathogen Phytophthora infestans and the early blight pathogen Alternaria solani induced hypersensitive response-like cell death accompanied by ROS production at the infection sites of transgenic plants. Transgenic plants showed resistance to the near-obligate hemibiotrophic pathogen P. infestans and, by contrast, increased susceptibility to the necrotrophic pathogen A. solani. These results indicate that RBOH-dependent ROS contribute to basal defense against near-obligate pathogens, but have a negative role in resistance or have a positive role in expansion of disease lesions caused by necrotrophic pathogens.
机译：马铃薯（Soianum tuberosum）钙依赖性蛋白激酶（StCDPKS）已显示磷酸化质膜RBOH（呼吸爆发氧化酶同系物）蛋白的N端区域，并参与StRBOHB介导的活性氧（ROS）爆发。组成型活性形式StCDPKSVK为防御反应中RBOH的功能获得分析提供了有用的工具。 StCDPK5-和StCDPK5VK-绿色荧光蛋白融合蛋白主要靶向质膜，StCDPKSVK的条件表达激活了StRBOHA-D。通过双分子荧光互补测定法证实了相互作用。我们在病原体诱导型启动子的控制下生成了含有StCDPK5VK的转基因马铃薯植株，以研究ROS爆发对枯萎病原体防御反应的作用。晚疫病病原菌疫霉和早疫病病原体链霉菌的强毒分离株在转基因植物的感染部位诱导了超敏反应样细胞死亡，并伴随着ROS的产生。转基因植物显示出对近专性半生营养病原体致病疫霉的抗性，相反，对坏死性病原体A. solani的敏感性增加。这些结果表明，依赖RBOH的ROS有助于抵抗近专性病原体的基础防御，但是在抵抗性中具有负作用，或者在由坏死性病原体引起的疾病损害扩展中具有正作用。

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