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首页> 外文期刊>The New Phytologist >SHORT INTERNODES/STYLISH genes, regulators of auxin biosynthesis, are involved in leaf vein development in Arabidopsis thaliana
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SHORT INTERNODES/STYLISH genes, regulators of auxin biosynthesis, are involved in leaf vein development in Arabidopsis thaliana

机译:生长激素生物合成的调节子,短INTERNODES / STYLISH基因,参与拟南芥叶静脉的发育

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摘要

Leaves depend on highly developed venation systems to collect fixed carbon for transport and to distribute water. We hypothesized that local regulation of auxin biosynthesis plays a role in vein development. To this effect, we assessed the role of theSHORT INTERNODES/ STYLISH (SHI/STY) gene family, zinc-finger transcription factors linked to regulation of auxin biosynthesis, in Arabidopsis thaliana leaf vein development. Gene functions were assessed by a combination of high-resolution spatio-temporal expression analysis of promoter-marker lines and phenotypic analysis of plants homozygous for single and multiple mutant combinations. The SHI/STY genes showed expression patterns with variations on a common theme of activity in incipient and developing cotyledon and leaf primordia, narrowing to apices and hydathode regions. Mutant analysis of single to quintuple mutant combinations revealed dose-dependent defects in vein patterning affecting multiple vein traits, most notably in cotyledons. Here we demonstrate that local regulation of auxin biosynthesis is an important aspect ofleaf vein development. Our findings also support a model in which auxin synthesized at the periphery of primordia affects vein development.
机译:叶子依靠高度发达的通风系统来收集固定的碳用于运输和分配水。我们假设生长素生物合成的局部调节在静脉发育中起作用。为了达到这一效果,我们评估了拟南芥叶静脉发育中SHORT INTERNODES / STYLISH(SHI / STY)基因家族,与生长素生物合成调控相关的锌指转录因子的作用。通过对启动子-标记系的高分辨率时空表达分析和对单个和多个突变体组合纯合的植物的表型分析的组合来评估基因功能。 SHI / STY基因在初生和发育中的子叶和叶片原基中表现出具有共同活动主题的表达模式,并变窄到顶端和透明膜区域。对单突变体到五重突变体组合的突变分析显示,静脉模式中的剂量依赖性缺陷会影响多种静脉特征,最明显的是子叶。在这里,我们证明了生长素生物合成的局部调节是叶静脉发育的重要方面。我们的发现还支持一种模型,其中在原基周围合成的生长素会影响静脉发育。

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