...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The journals of gerontology.Series A. Biological sciences and medical sciences >Dietary restriction of Caenorhabditis elegans by axenic culture reflects nutritional requirement for constituents provided by metabolically active microbes.
【24h】

Dietary restriction of Caenorhabditis elegans by axenic culture reflects nutritional requirement for constituents provided by metabolically active microbes.

机译：厌食培养对秀丽隐杆线虫的饮食限制反映了代谢活性微生物所提供成分的营养需求。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In Caenorhabditis elegans, several manipulations that affect nutrition slow development, reduce fecundity, and increase life span. These are viewed as dietary restriction (DR) and include culture in semidefined, nutrient-rich liquid medium that is axenic (i.e., there is no microbial food source). Here we describe convenient ways to exert DR by culture on agar plates containing axenic medium. We used these to explore whether effects of axenic culture really reflect DR. Our results imply that major nutrient components of axenic medium, and overall caloric content, are not limiting for life span. However, adding growth-arrested Escherichia coli as an additional food source rescued the effects of axenic culture. We then sought to identify the component of E. coli that is critical for normal C. elegans nutrition using add-back experiments. Our results suggest that C. elegans has a nutritional requirement for live, metabolically active microbes or, possibly, an unidentified, heat-labile, nonsoluble componentpresent in live microbes.
机译：在秀丽隐杆线虫中，几种影响营养的操作会减缓发育，减少繁殖力并延长寿命。这些被视为饮食限制（DR），包括在半定义的，营养丰富的液体培养基中进行培养，该培养基是无菌的（即没有微生物食物来源）。在这里，我们描述了通过在含有阿森烯培养基的琼脂平板上培养来施加DR的便捷方法。我们用这些来探讨焦虑症文化的影响是否真正反映了DR。我们的研究结果表明，轴心培养基的主要营养成分以及总热量均不受寿命的限制。但是，添加生长停滞的大肠杆菌作为额外的食物来源可以挽救树苗培养的影响。然后，我们尝试使用回加实验来鉴定对正常秀丽隐杆线虫营养至关重要的大肠杆菌成分。我们的研究结果表明，秀丽隐杆线虫对活的，具有代谢活性的微生物或活的微生物中存在的不确定的，热不稳定的，不溶性成分具有营养需求。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号