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首页> 外文期刊>The journals of gerontology.Series A. Biological sciences and medical sciences >Proteomic analysis of nitrated and 4-hydroxy-2-nonenal-modified serum proteins during aging.
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Proteomic analysis of nitrated and 4-hydroxy-2-nonenal-modified serum proteins during aging.

机译：蛋白质组学分析硝化和4-羟基-2-壬烯修饰的血清蛋白老化过程中。

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摘要

Using proteomic techniques, we investigated peroxynitrite (ONOO-) and 4-hydroxy-2-nonenal (HNE) modified serum proteins from young and old Fischer 344 rats. Two-dimensional gel electrophoresis/western blot analysis of nitrotyrosine and HNE-histidine revealed that serum proteins were differentially modified by ONOO- and HNE. Among them, 16 of the modified proteins, identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), are involved in blood coagulation, lipid transport, blood pressure regulation, and protease inhibition. Furthermore, nitration and HNE adduction were found to increase with age, lending support to the oxidative stress hypothesis of aging. Our data showed that proteomic techniques can be valuable tools in the study of protein profiling modifications during aging.
机译：使用蛋白质组学技术，我们研究了来自老幼Fischer 344大鼠的过氧亚硝酸盐（ONOO-）和4-羟基-2-壬烯醛（HNE）修饰的血清蛋白。硝基酪氨酸和HNE-组氨酸的二维凝胶电泳/ western印迹分析表明，ONOO-和HNE对血清蛋白进行了差异修饰。其中，通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）鉴定的16种修饰蛋白与血液凝固，脂质转运，血压调节和蛋白酶抑制有关。此外，发现硝化作用和HNE加合随着年龄的增长而增加，这为衰老的氧化应激假设提供了支持。我们的数据表明，蛋白质组学技术可能是研究衰老过程中蛋白质轮廓分析的有价值的工具。

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