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首页> 外文期刊>The Lichenologist >Detection of paralogous polyketide synthase genes in Parmeliaceaeby specific primers
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Detection of paralogous polyketide synthase genes in Parmeliaceaeby specific primers

机译：特异引物检测梅花科中的同源聚酮化合物合酶基因

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摘要

A first assessment of paralogy in non-reducing polyketide synthases of Parmeliaceae is presented. Primers which are specific to the keto-acyl synthase domain were used to amplify gene fragments of putative non-reducing polyketide synthases from various representatives of the family. The corresponding sequences were analysed together with a selection of known polyketide synthase genes from other fungi, including lichenized fungi. The results suggest that genes from Parmeliaceae represent at least 6 paralogs. Their different positions in the tree partly correlate with the variable presence of spliceosomal introns at particular positions in the gene fragments. Because only one paralog could be unambiguously detected in each species by direct sequencingof PCR products with this approach, we tested the applicability of clade-specific primers, designed by using orthologous signature sequences. With these primers more paralogs could be detected from the same DNA extract in a number of species, but certain paralogs were consistently not amplified in these species. The paralog-specific primer approach can potentially be used for a rapid screening of PKS genes from a broader range of lichen fungi.
机译：提出了对伞形科的非还原性聚酮化合物合酶的寄生虫的首次评估。对酮-酰基合酶结构域特异的引物用于扩增来自该家族各个代表的推定的非还原性聚酮化合物合酶的基因片段。分析了相应的序列，并从其他真菌（包括地衣真菌）中选择了已知的聚酮化合物合酶基因。结果表明，来自伞形科的基因代表至少6个旁系同源物。它们在树中的不同位置与基因片段中特定位置的剪接内含子的可变存在部分相关。因为通过这种方法通过PCR产物的直接测序在每个物种中只能清楚地检测到一个旁系同源物，所以我们测试了通过使用直系同源签名序列设计的进化枝特异性引物的适用性。使用这些引物，可以从许多物种的同一DNA提取物中检测到更多的旁系同源物，但是某些旁系同源物在这些物种中始终未扩增。同源物特异性引物方法可潜在地用于从更广泛的地衣真菌中快速筛选PKS基因。

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