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首页> 外文期刊>Biomaterials Science >Folic acid conjugated magnetic iron oxide nanoparticles for nondestructive separation and detection of ovarian cancer cells from whole blood
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Folic acid conjugated magnetic iron oxide nanoparticles for nondestructive separation and detection of ovarian cancer cells from whole blood

机译：叶酸共轭磁性氧化铁纳米颗粒用于无损分离和检测全血卵巢癌细胞

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摘要

Because of the lack of early screening strategies, ovarian cancer is the most deadly cause of gynecologic malignancies. This paper describes an effective method for the separation and detection of ovarian cancer cells from female whole blood, using folic acid (FA) conjugated magnetic iron oxide nanoparticles (IO-FA nanoparticles). The IO nanoparticles were synthesized by thermal decomposition and then covalently conjugated with FA. The IO-FA nanoparticles were stably attached to the surface of ovarian cancer cells by coupling to the over-expressed folate receptor (FR), thereby making the cells magnetic. These "magnetic cells" were separated from the complex blood matrix without destruction under a magnetic field. The separation efficiency was as high as 61.3% when the abundance of spiked ovarian cancer SKOV3 cells was as low as 5 x 10 (5)%. We also successfully detected five (5) out of ten (10) metastatic ovarian cancer patients' whole blood. This study suggested the feasibility of early detecting of metastatic ovarian cancer cells, which may potentially improve the ovarian cancers patients' overall survival rate for clinical applications.
机译：由于缺乏早期筛查策略，卵巢癌是妇科恶性肿瘤的最致命原因。本文介绍了一种有效的方法，该方法使用叶酸（FA）共轭磁性氧化铁纳米粒子（IO-FA纳米粒子）从女性全血中分离和检测卵巢癌细胞。通过热分解合成IO纳米颗粒，然后与FA共价缀合。 IO-FA纳米颗粒通过与过度表达的叶酸受体（FR）偶联而稳定地附着于卵巢癌细胞的表面，从而使细胞具有磁性。这些“磁性细胞”从复杂的血液基质中分离出来，在磁场下没有被破坏。当加标的卵巢癌SKOV3细胞的丰度低至5 x 10（5）％时，分离效率高达61.3％。我们还成功地在十（10）个转移性卵巢癌患者的全血中检测出五（5）个。这项研究提出了早期检测转移性卵巢癌细胞的可行性，这可能潜在地提高卵巢癌患者在临床应用中的总体存活率。

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