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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Microtia chondrocytes as a donor source for tissue-engineered cartilage.
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Microtia chondrocytes as a donor source for tissue-engineered cartilage.

机译：小耳软骨细胞作为组织工程软骨的供体来源。

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OBJECTIVES/HYPOTHESIS: Current surgical techniques for the correction of microtia are challenging. Research in the field of tissue engineering is providing insight into chondrocyte behavior for a possible future treatment of microtia. The authors wished to evaluate the biological potential of chondrocytes isolated from microtia cartilage as compared with normal auricular cartilage as a source of tissue-engineered cartilage. STUDY DESIGN: A comparative research design to study the potential of microtia cartilage chondrocytes with normal auricular chondrocytes as a source of tissue-engineered cartilage. METHODS: Cartilage specimens from 12 pediatric patients (six normal auricular specimens and six auricular specimens with microtia) were obtained. The chondrocytes were isolated and cultured in vitro; chondrocyte number was increased by passaging. Each type of cell was implanted in nude mice to generate tissue-engineered cartilage. Eight weeks after implantation the specimens were dissected and removed. Results were compared between the normal auricular and microtia specimens in regard to cell number expansion in vitro and generation of tissue-engineered cartilage in vivo. RESULTS: An initial mean cell number of 150,000 cells in each group (normal and microtia) increased to an average cell number of 120 million cells/mL in the normal and 130 million cells in the microtia subgroups, respectively, at the end of the second passage. Histologically, both types of chondrocytes generated normal elastic cartilage. CONCLUSION: The study demonstrated the potential of cells isolated from microtia cartilage to generate tissue-engineered cartilage. Microtia cartilage represents an important additional donor source for the possible generation of a human tissue-engineered auricle.
机译：目的/假设：目前用于矫正小眼症的外科手术技术具有挑战性。组织工程领域的研究为软骨细胞行为的深入了解提供了可能，可能在未来治疗小口疮。作者希望评估与作为组织工程软骨来源的正常耳软骨相比，从小口软骨分离的软骨细胞的生物学潜力。研究设计：一项对比研究设计，旨在研究小耳软骨软骨细胞与正常耳软骨细胞作为组织工程软骨来源的潜力。方法：从12名儿科患者的软骨标本中（6个正常耳标和6个耳镜标本）。分离软骨细胞并在体外培养;传代使软骨细胞数量增加。每种类型的细胞都植入裸鼠体内，以产生组织工程软骨。植入后八周，将标本解剖并取出。比较了正常耳廓和小耳标本在体外细胞数量扩增和体内组织工程软骨生成方面的结果。结果：第二组结束时，每组（正常和小口症）的初始平均细胞数为150,000个细胞，正常组和小口症亚组的平均平均细胞数分别为1.2亿个细胞/mL，1.3亿个细胞/ mL。通道。组织学上，两种类型的软骨细胞均产生正常的弹性软骨。结论：该研究证明了从小口软骨分离出的细胞产生组织工程软骨的潜力。小耳软骨代表了可能产生的人类组织工程化耳廓的重要的额外供体来源。

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