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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Expression of matrix metalloproteinases in supraglottic carcinoma and its clinical implication for estimating lymph node metastases.
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Expression of matrix metalloproteinases in supraglottic carcinoma and its clinical implication for estimating lymph node metastases.

机译：基质金属蛋白酶在声门上癌中的表达及其对淋巴结转移的临床意义。

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OBJECTIVE: Twenty to 30% of clinically node-negative patients with squamous cell carcinoma (SCC) of supraglottic larynx were pathologically confirmed with occult lymph node metastasis after operation. Therefore, preoperative judgment on the neck node metastasis should be very important for making an appropriate surgical plan. The purpose of this study is to explore the role of matrix metalloprotineases (MMPs) in the metastatic potential of supraglottic carcinoma in this regard. STUDY DESIGN: Examination of MMPs expression in supraglottic carcinoma and establishment of the mathematic model for diagnosis of occult lymph node metastases in a prospective manner. METHODS: Thirty-two patients with supraglottic SCC underwent supraglottic horizontal partial laryngectomy and neck dissection, and the samples of primary cancer and the corresponding adjacent non-neoplastic tissues were evaluated for both mRNA and protein expression of MMP-1, MMP-2, MMP-7, MMP-8, MMP-9, and MMP-10 using reverse transcriptase polymerase chain reaction (RT-PCR) and immunohistochemistry. In addition, cervical lymph nodes were examined by routine histopathology to determine whether there was any evidences of node metastasis. RT-PCR and immunohistochemical data were analyzed by multivariate logistic regression, and then two mathematical models were established according to the multivariate logistic regression analysis. RESULTS: Among the six kinds of MMPs, both mRNA and protein expression of MMP-2, MMP-7, and MMP-9 were found up-regulated in more than 63% of the tumor tissues as compared with the adjacent non-neoplastic tissues, and this was significantly correlated with lymph node metastasis (all P < .05). Twenty patients, including seven cases with N0, were pathologically confirmed as having cervical lymph node metastasis. Using RT-PCR and immunohistochemical data of MMP-2, MMP-7, and MMP-9, two mathematic models were tested, and their diagnostic efficiencies for lymph node metastasis were 84% and 88%, respectively. CONCLUSION: Expressions of MMP-2, MMP-7, and MMP-9 are up-regulated in supraglottic carcinoma tissues, suggesting that MMPs may be useful in the detection of occult lymph node metastasis but that more research is needed.
机译：目的：经病理学证实，临床上有20％至30％的声门上喉鳞状细胞癌（SCC）阴性患者术后发生隐匿性淋巴结转移。因此，术前对颈部淋巴结转移的判断对于制定适当的手术计划非常重要。这项研究的目的是探讨基质金属蛋白酶在这方面在声门上癌转移潜力中的作用。研究设计：检查声门上癌中MMPs的表达，并建立前瞻性诊断隐匿性淋巴结转移的数学模型。方法：对32例声门上SCC患者行声门上水平部分喉切除术和颈淋巴结清扫术，并评估原发癌及相应的邻近非肿瘤组织的MMP-1，MMP-2，MMP的mRNA和蛋白表达。 -7，MMP-8，MMP-9和MMP-10使用逆转录聚合酶链反应（RT-PCR）和免疫组化。另外，通过常规的组织病理学检查宫颈淋巴结，以确定是否有淋巴结转移的证据。通过多元logistic回归分析RT-PCR和免疫组化数据，然后根据多元logistic回归分析建立两个数学模型。结果：在六种MMPs中，与邻近的非肿瘤组织相比，在超过63％的肿瘤组织中MMP-2，MMP-7和MMP-9的mRNA和蛋白表达均被上调。 ，这与淋巴结转移密切相关（所有P <.05）。病理证实20例患者（包括7例N0病例）有颈部淋巴结转移。使用RT-PCR和MMP-2，MMP-7和MMP-9的免疫组化数据，测试了两个数学模型，它们对淋巴结转移的诊断效率分别为84％和88％。结论：声门上癌组织中MMP-2，MMP-7和MMP-9的表达上调，提示MMPs可用于隐匿性淋巴结转移的检测，但尚需进一步研究。

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