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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Expression of MUC5AC mRNA in the goblet cells of human nasal mucosa.
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Expression of MUC5AC mRNA in the goblet cells of human nasal mucosa.

机译：MUC5AC mRNA在人鼻黏膜杯状细胞中的表达。

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OBJECTIVES/HYPOTHESIS: Mucus hypersecretion is a common feature in chronic sinusitis with polyps. Because mucus hypersecretion is commonly accompanied by goblet cell hyperplasia, it is important to identify which mucin gene mRNAs are expressed in the goblet cells of the surface epithelium in the human airway. This study aims to investigate the pattern of expression of MUC5AC messenger RNA (mRNA) in the goblet cells of human nasal mucosa. METHODS: Six nasal polyps, five inferior turbinate mucosa specimens, and three normal-appearing mucosa specimens of the posterior ethmoid sinus were obtained. Each of the specimens was cut into 10-microm-thick serial frozen sections, and in situ hybridization of MUC5AC mRNA was performed with an oligonucleotide probe. Alcian blue (pH 2.5)-periodic acid-Schiff (AB-PAS) staining was performed on the serial sections. RESULTS: In human nasal polyps, MUC5AC mRNA was expressed in the cytoplasm of most of the goblet cells. However, in the inferior turbinate, MUC5AC mRNA was expressed in only some of the goblet cells. On the contrary, in the normal-appearing mucosa of the posterior ethmoid sinus, MUC5AC mRNA was barely expressed in the goblet cells. Furthermore, MUC5AC mRNA was mainly expressed in some of the PAS-positive goblet cells. CONCLUSIONS: Only a portion of the goblet cells in the human nasal mucosa expressed MUC5AC mRNA. This result suggests that surface goblet cells might have other mucin genes, in addition to MUC2 and MUC5AC.
机译：目的/假设：粘液分泌过多是慢性鼻窦炎伴息肉的常见特征。由于粘液分泌过多通常伴随杯状细胞增生，因此重要的是鉴定在人气道表面上皮的杯状细胞中表达哪些粘蛋白基因mRNA。这项研究旨在调查人鼻黏膜杯状细胞中MUC5AC信使RNA（mRNA）的表达模式。方法：获得6个鼻息肉，5个下鼻甲粘膜标本和3个筛骨后窦正常出现的粘膜标本。将每个样品切成10微米厚的连续冷冻切片，并用寡核苷酸探针进行MUC5AC mRNA的原位杂交。在连续切片上进行了阿尔辛蓝（pH 2.5）-高碘酸席夫（AB-PAS）染色。结果：在人的鼻息肉中，MUC5AC mRNA在大多数杯状细胞的细胞质中表达。然而，在下鼻甲中，MUC5AC mRNA仅在一些杯状细胞中表达。相反，在筛骨后窦的正常出现的粘膜中，MUC5AC mRNA在杯状细胞中几乎不表达。此外，MUC5AC mRNA主要在某些PAS阳性杯状细胞中表达。结论：人鼻粘膜中只有一部分杯状细胞表达MUC5AC mRNA。该结果表明除了MUC2和MUC5AC之外，表面杯状细胞可能还具有其他粘蛋白基因。

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