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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Effects of a nerve-muscle pedicle on the denervated rat thyroarytenoid muscle.
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Effects of a nerve-muscle pedicle on the denervated rat thyroarytenoid muscle.

机译：神经肌肉蒂对失神经的大鼠甲状腺素类肌肉的影响。

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OBJECTIVES: To evaluate the effects of the nerve-muscle pedicle (NMP) method on the rat thyroarytenoid (TA) muscle after transection of the recurrent laryngeal nerve (RLN). STUDY DESIGN: Quantitative histologic assessment of the TA muscle after NMP implantation. METHODS: Thirty-six Wistar rats were divided into two groups: animals subjected to transection of the left RLN alone (DNV group) and animals subjected to transection of the left RLN followed by immediate transplantation of a NMP flap containing the sternohyoid (SH) muscle and ansa cervicalis nerve branch (NMP group). Animals were killed 2, 4, and 10 weeks after the treatments. The TA muscle stained with hematoxylin-eosin was evaluated quantitatively. The pre- and postsynaptic structures of the neuromuscular junction (NMJ) in the TA muscle were analyzed histochemically. The myosin heavy chain (MyHC) isoforms were evaluated using immunohistochemistry and reverse-transcriptase polymerase chain reaction (RT-PCR). RESULTS: In the NMP group, the TA muscle fiber recovered to almost normal at 10 weeks, and the ratio of the number of synaptophysin-positive nerve terminals to that of alpha-bungarotoxin-positive acetylcholine receptors recovered to 79.8 +/- 11.8% (P < .05, compared with the control). Immunohistochemistry and the RT-PCR method after laser capture microdissection revealed the expression of MyHC isoforms type 2B and type 2A; the latter was detected in the SH muscle but not in the normal or denervated TA muscle. CONCLUSION: The NMP method was effective for recovering from the atrophic changes of the TA muscle after transection of the RLN. This was attributed to successful reinnervation by reconstruction of the NMJ, which might change MyHC isoform expression.
机译：目的：评价神经肌肉蒂（NMP）方法对喉返神经（RLN）横断后大鼠甲状腺素（TA）肌肉的影响。研究设计：NMP植入后TA肌肉的定量组织学评估。方法：将36只Wistar大鼠分为两组：单独切除左RLN的动物（DNV组）和切除左RLN的动物，然后立即移植含有胸骨舌骨（SH）肌肉的NMP皮瓣和颈an神经支（NMP组）。治疗后2、4和10周将动物处死。定量评估苏木精-曙红染色的TA肌肉。组织化学分析了TA肌肉中神经肌肉接头（NMJ）的突触前和突触后结构。使用免疫组织化学和逆转录酶聚合酶链反应（RT-PCR）评估了肌球蛋白重链（MyHC）亚型。结果：在NMP组中，TA肌纤维在10周后恢复到几乎正常，并且突触素阳性神经末梢数目与α-真菌毒素阳性乙酰胆碱受体数目之比恢复为79.8 +/- 11.8％（与对照相比，P <.05）。激光捕获显微切割后的免疫组织化学和RT-PCR方法显示了2B型和2A型MyHC亚型的表达。后者是在SH肌肉中检测到的，而在正常或失神经的TA肌肉中没有检测到。结论：NMP法可有效切除RLN后，从TA肌萎缩性变化中恢复。这归因于通过重建NMJ成功进行的神经支配，这可能会改变MyHC亚型的表达。

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