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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Nasal vaccination with CpG oligodeoxynucleotide induces protective immunity against non-typeable Haemophilus influenzae in the nasopharynx.
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Nasal vaccination with CpG oligodeoxynucleotide induces protective immunity against non-typeable Haemophilus influenzae in the nasopharynx.

机译：鼻腔接种CpG寡脱氧核苷酸可诱导针对鼻咽中不可分型流感嗜血杆菌的保护性免疫。

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OBJECTIVES: Nasal vaccination is an effective therapeutic regimen for preventing otitis media. Since cholera toxin (CT) is toxic, an alternative adjuvant is required for the development of a nasal vaccine. The efficacy of CpG oligodeoxynucleotide (ODN) as a mucosal adjuvant was examined. METHODS: Mice were immunized intranasally with P6 protein of non-typeable Haemophilus influenzae (NTHi) and adjuvant, CT, or CpG ODN, and P6-specific antibody responses were examined. The expression of P6-specific cytokine mRNA in splenic CD4 T cells was also determined. In addition, NTHi challenges were performed and the NTHi was quantified in nasal washes. RESULTS: P6-specific IgA in nasal wash and serum IgG titers were elevated significantly after nasal immunization. The IgG1/IgG2a ratio in serum from P6+CpG-immunized mice was less than that of P6+CT-immunized mice. Although IL-6 was expression similarly in both groups, IFN-gamma expression was greater in P6+CpG-immunized mice than in P6+CT-immunized mice. Enhanced clearance of NTHi from the nasopharynx was also shown equally in both groups. CONCLUSION: These results indicate that CpG ODN might be an effective mucosal adjuvant, acting by mechanisms that are different from CT. These findings suggest that nasal vaccination with P6 and CpG ODN might be an effective regimen for the induction of NTHi-specific protective immunity.
机译：目的：鼻疫苗接种是预防中耳炎的有效治疗方案。由于霍乱毒素（CT）是有毒的，因此开发鼻疫苗需要替代佐剂。检查了CpG寡脱氧核苷酸（ODN）作为粘膜佐剂的功效。方法：用不可分型流感嗜血杆菌（NTHi）的P6蛋白和佐剂，CT或CpG ODN鼻内免疫小鼠，并检查P6特异性抗体反应。还确定了脾CD4 T细胞中P6特异性细胞因子mRNA的表达。另外，进行了NTHi挑战，并且在鼻洗液中定量了NTHi。结果：经鼻免疫后，鼻洗液中P6特异性IgA明显升高，血清IgG滴度明显升高。 P6 + CpG免疫小鼠的血清中IgG1 / IgG2a的比例小于P6 + CT免疫小鼠的IgG1 / IgG2a的比例。尽管两组中IL-6的表达相似，但P6 + CpG免疫的小鼠的IFN-γ表达要高于P6 + CT免疫的小鼠。在两组中，NTHi从鼻咽的清除率也有所提高。结论：这些结果表明，CpG ODN可能是一种有效的粘膜佐剂，其作用机理不同于CT。这些发现表明，鼻腔接种P6和CpG ODN疫苗可能是诱导NTHi特异性保护性免疫的有效方案。

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