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首页> 外文期刊>The Laryngoscope: A Medical Journal for Clinical and Research Contributions in Otolaryngology, Head and Neck Medicine and Surgery, Facial Plastic and Reconstructive Surgery .. >Overexpression of scatter factor and its receptor (c-met) in oral squamous cell carcinoma.
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Overexpression of scatter factor and its receptor (c-met) in oral squamous cell carcinoma.

机译：口腔鳞状细胞癌中散射因子及其受体（c-met）的过表达。

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HYPOTHESIS: Scatter factor (SF) is a pleiotropic growth factor that recently has been shown to induce epithelial cell proliferation, random motility, and invasion via interaction with its receptor, a tyrosine kinase encoded by the c-met proto-oncogene. Studies involving a variety of solid tumors have suggested that overexpression of the SF/c-met ligand-receptor pair is associated with the acquisition of a malignant phenotype. We hypothesize that SF and c-met are overexpressed in epithelial malignancies of the head and neck including squamous cell carcinoma (SCC) of the oral cavity. STUDY DESIGN: Immunohistochemical staining of randomly selected normal, dysplastic, and malignant oral tissues. METHODS: Formalin-fixed, paraffin-embedded tissues were obtained from the Department of Oral Pathology at Shands Hospital (University of Florida), Gainesville, Florida. Examples of mild dysplasia, severe dysplasia, well-differentiated SCC, moderately differentiated SCC, and poorly differentiated SCC were randomly selected from the dictated reports of one of two staff oral pathologists. Histologically normal margins of each specimen served as normal controls. The tissues were immunohistochemically stained using commercially available antibodies against SF and c-met. Appropriate negative controls were run with each batch to ensure staining specificity. Evaluation of staining intensity was carried out using a computerized image analysis system. A one-way analysis of variance (ANOVA) with pairwise multiple-comparison procedures (Fisher method) was used to analyze the data. RESULTS: Statistically significant differences (P < .0001) in the intensity of staining were noted between the malignant and normal and the malignant and dysplastic tissues for both SF and c-met. No differences were appreciated when staining of normal and dysplastic sections of the SF-stained tissue were compared. CONCLUSIONS: The results suggest that the SF/c-met ligand-receptor pair is overexpressed in SCC of the oral cavity.
机译：假设：散射因子（SF）是一种多效性生长因子，最近已证明通过与其受体（由c-met原癌基因编码的酪氨酸激酶）相互作用，诱导上皮细胞增殖，随机运动和侵袭。涉及多种实体瘤的研究表明，SF / c-met配体-受体对的过度表达与恶性表型的获得有关。我们假设SF和c-met在头和颈部的上皮恶性肿瘤（包括口腔鳞状细胞癌（SCC））中过表达。研究设计：随机选择的正常，发育异常和恶性口腔组织的免疫组织化学染色。方法：福尔马林固定，石蜡包埋的组织获自佛罗里达州盖恩斯维尔市Shands医院（佛罗里达大学）口腔病理学系。从两名口腔病理学家之一的指定报告中随机选择轻度发育不良，严重发育异常，SCC高分化，SCC中分化和SCC低分化的例子。每个标本的组织学正常边缘用作正常对照。使用可商购的针对SF和c-met的抗体对组织进行免疫组织化学染色。每批均进行适当的阴性对照，以确保染色特异性。使用计算机图像分析系统进行染色强度的评估。使用成对的多重比较程序（Fisher方法）的单向方差分析（ANOVA）来分析数据。结果：SF和c-met的恶性和正常组织与恶性和增生组织之间的染色强度在统计学上有显着差异（P <.0001）。比较SF染色组织的正常切片和增生切片的染色时，没有差异。结论：结果表明，SF / c-met配体-受体对在口腔SCC中过表达。

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