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首页> 外文期刊>The Prostate >Differential expression of matrix metalloproteinases and their tissue inhibitors in human primary cultured prostatic cells and malignant prostate cell lines.
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Differential expression of matrix metalloproteinases and their tissue inhibitors in human primary cultured prostatic cells and malignant prostate cell lines.

机译：基质金属蛋白酶及其组织抑制剂在人原代培养的前列腺细胞和恶性前列腺细胞系中的差异表达。

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BACKGROUND: The aim was to investigate the expression of matrix metalloproteinases (MMPs), membrane type MMPs (MT-MMPs), and their inhibitors (TIMPs) in human primary cultured prostatic cells and malignant prostate cell lines. METHODS: Reverse transcription-polymerase chain reaction-based measurements of the mRNA levels of MMP-2, MMP-7, MT1-MMP, MT3-MMP, TIMP-1, and TIMP-2 in relation to the house-keeping gene glyceraldehyde phosphate dehydrogenase were performed in cancerous and non-cancerous prostatic tissue samples, in primary cell cultures of epithelial cells, in both fibroblasts, and smooth-muscle cells as stromal cells, and in the human malignant prostatic cell lines DU-145, LNCaP, and PC-3. RESULTS: MMP-2 was mainly expressed in the stromal cells and MMP-7 showed their highest values in the epithelial cells. MT1-MMP, MT3-MMP, TIMP-1, and TIMP-2 were found both in the stromal and in the epithelial cells, but there were some differences between the expressions in fibroblasts and smooth-muscle cells. Different expressions were also observed between the cells deriving from the primary cell cultures, the benign cell line BPH-1, and the malignant cell lines LNCaP, D-145, and PC-3. CONCLUSIONS: These exemplary results concerning different expressions of MMPs and TIMPs in cells from prostatic tissue suggest that a better insight into changes observed in prostatic tissue needs studies on cells cultured from the tissue. Copyright 2002 Wiley-Liss, Inc.
机译：背景：目的是研究基质金属蛋白酶（MMPs），膜型MMPs（MT-MMPs）及其抑制剂（TIMPs）在人原代培养的前列腺细胞和恶性前列腺细胞系中的表达。方法：基于逆转录-聚合酶链反应的测量与管家基因磷酸甘油醛相关的MMP-2，MMP-7，MT1-MMP，MT3-MMP，TIMP-1和TIMP-2的mRNA水平脱氢酶是在癌性和非癌性前列腺组织样品中，在上皮细胞的原代细胞培养物中，在成纤维细胞和平滑肌细胞（作为基质细胞）以及人恶性前列腺细胞系DU-145，LNCaP和PC中进行的-3。结果：MMP-2主要在基质细胞中表达，MMP-7在上皮细胞中表达最高。在基质细胞和上皮细胞中均发现了MT1-MMP，MT3-MMP，TIMP-1和TIMP-2，但在成纤维细胞和平滑肌细胞中的表达有所不同。在原代细胞培养的细胞，良性细胞系BPH-1和恶性细胞系LNCaP，D-145和PC-3之间也观察到了不同的表达。结论：这些关于前列腺组织细胞中MMPs和TIMPs不同表达的示例性结果表明，对前列腺组织中观察到的变化的更好了解需要对从组织培养的细胞进行研究。版权所有2002 Wiley-Liss，Inc.

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