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首页> 外文期刊>The Progressive Fish-Culturist >CHANGES IN MORPHOLOGY, PHYSIOLOGY, METABOLISM, AND FERTILIZATION CAPACITY OF RAINBOW TROUT SEMEN FOLLOWING CRYOPRESERVATION
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CHANGES IN MORPHOLOGY, PHYSIOLOGY, METABOLISM, AND FERTILIZATION CAPACITY OF RAINBOW TROUT SEMEN FOLLOWING CRYOPRESERVATION

机译：低温保存后虹鳟精液形态，生理，代谢和施肥能力的变化

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摘要

Alterations in morphology, physiology, metabolism, and fertilization capacity of the semen of rainbow trout Oncorhynchus mykiss after cryopreservation were investigated. In frozen-thawed semen, only about 10-20% of the spermatozoa had an unchanged structure that was similar to untreated spermatozoa; about 20-40% showed intensive signs of swelling of the head and midpiece regions and of the mitochondria. The remaining spermatozoa were damaged in some way. When compared with spermatozoa in untreated semen, the percentage of immotile spermatozoa in frozen-thawed semen was significantly higher, and the rate of motile spermatozoa was decreased. In fertilization solution, the motility of spermatozoa was mainly circular before cryopreservation and linear after cryopreservation. The swimming velocity of spermatozoa did not differ between untreated and frozen-thawed semen. Activities of adenylate kinase and of pyruvate kinase in untreated semen and frozen-thawed semen did not differ significantly. Activities of isocitrate dehydrogenase, malate dehydrogenase kinase, L-lactate dehydrogenase, and ATPase were significantly lower in frozen-thawed semen than in untreated semen. Also, creatine phosphate and ATP levels were significantly lower in frozen-thawed semen than in untreated semen. The ADP, lactate, pyruvate, glucose, and triglyceride levels of untreated and frozen-thawed semen did not significantly differ. At ratios of 3 x 10(6) spermatozoa/egg or greater, the fertilization rate of frozen-thawed semen was similar to that of untreated semen, but at lower sperm : egg ratios, fertilization rate was significantly reduced.
机译：冷冻后虹鳟Onkihynchus mykiss精液的形态，生理，代谢和受精能力的变化进行了调查。在冷冻解冻的精液中，只有约10-20％的精子具有与未经处理的精子相似的不变结构。约有20-40％的人表现出明显的头，中段和线粒体肿胀迹象。剩余的精子以某种方式受损。与未经处理的精液中的精子相比，冷冻解冻精液中不活动精子的百分比显着更高，而活动精子的比率则下降。在施肥溶液中，精子的活力在冷冻前主要为圆形，在冷冻后为线性。未经处理和冻融的精液的精子游动速度没有差异。未经处理的精液和冻融精液中腺苷酸激酶和丙酮酸激酶的活性没有显着差异。冻融精液中异柠檬酸脱氢酶，苹果酸脱氢酶激酶，L-乳酸脱氢酶和ATPase的活性明显低于未处理精液。另外，冻融精液中的磷酸肌酸和ATP水平明显低于未处理精液。未经处理和冻融的精液的ADP，乳酸，丙酮酸，葡萄糖和甘油三酸酯水平无显着差异。当精子比率为3 x 10（6）或更高时，冷冻解冻的精液的受精率与未处理的精液相似，但精子与卵的比率较低时，受精率明显降低。

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