• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>General and comparative endocrinology >Effect of dilution in sperm maturation media and time of storage on sperm motility and fertilizing capacity of cryopreserved semen of sex-reversed female rainbow trout
【24h】

Effect of dilution in sperm maturation media and time of storage on sperm motility and fertilizing capacity of cryopreserved semen of sex-reversed female rainbow trout

机译：精子成熟培养基和储存时间对性倒装女性彩虹鳟鱼精子运动和施肥能力的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Masculinized females, also called neomales or sex-reversed females have a male phenotype but retain the female genotype (XX). Therefore, all spermatozoa produced in their functional testes carry an X chromosome, which is desired for the production of all-female rainbow trout populations. Semen of sex-reversed female rainbow trout is of low quality and in vitro maturation is required, which includes dilution of sperm suspensions with specially formulated maturation solutions. The aim of this study was to determine the effect of dilution in different maturation media on sperm quality (sperm motility characteristics and fertilizing capacity) of frozen/thawed sperm of sex-reversed female rainbow trout. The effect of time of post-thaw storage (0, 15, 60 and 120 min) on semen quality was also tested. Sperm motility parameters and fertilization rate at the eyed and hatching stages were assessed for post-thaw semen diluted in different media. The cryopreservation procedure resulted in high post-thaw sperm motility of about 57% and did not differ from fresh semen. Unexpectedly, maturation media decreased sperm activation capacity immediately after dilution; however, sperm motility increased over time. Fertilization rates of frozen/thawed semen were high (71-87%) and did not differ significantly between experimental variants at any of tested periods of storage. Our results demonstrated that the effect of the maturation media on frozen/thawed sperm is different from that of fresh sperm. The progressive increase in post-thaw sperm motility in maturation media can potentially be applied to routine hatchery practice. (C) 2016 Elsevier Inc. All rights reserved.
机译：男性化的女性，也称为NeoMales或性逆转的女性具有雄性表型，但保留女性基因型（XX）。因此，在其功能睾丸中产生的所有精子都携带X染色体，这是为生产全部女性彩虹鳟鱼的生产。性倒装雌性彩虹鳟鱼的精液具有低质量，需要体外成熟，这包括稀释具有特殊配制成熟溶液的精子悬浮液。本研究的目的是确定不同成熟培养基中的稀释对切冻/解冻母虹鳟鱼的精子质量（精子活力特征和施肥能力）的效果。还测试了解冻后储存时间（0,15,60和120分钟）的效果。对眼睛和孵化阶段的精子运动参数和施肥率进行评估，用于在不同培养基中稀释的解冻后液体。冷冻保存程序导致高温后精子活性约为57％，与新鲜精液没有不同。出乎意料的是，成熟培养介质在稀释后立即降低精子活化能力;然而，精子动力随着时间的推移而增加。冷冻/解冻精液的施肥率高（71-87％），在任何经过​​测试的储存期的实验变体之间没有显着差异。我们的研究结果表明，成熟培养基对冷冻/解冻精子的影响与新鲜精子的影响不同。可以应用于成熟培养基中解冻后精子运动的逐步增加来应用于常规孵化场实践。 （c）2016年Elsevier Inc.保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号