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首页> 外文期刊>The protein journal >Proteomic Analysis of the Defense Response of Wheat to the Powdery Mildew Fungus, Blumeria graminis f. sp tritici
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Proteomic Analysis of the Defense Response of Wheat to the Powdery Mildew Fungus, Blumeria graminis f. sp tritici

机译：小麦对白粉病真菌Blumeria graminis f。防御反应的蛋白质组学分析。小麦

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摘要

Powdery mildew of wheat is caused by Blumeria graminis f. sp. tritici (Bgt). Although many wheat cultivars resistant to this disease have been developed, little is known about their resistance mechanisms. The aim of this study was to identify proteins showing changes in abundance during the resistance response of the wheat line N0308 infected by Bgt. In two-dimensional electrophoresis analyses, 45 spots on the gels showed significant changes in abundance at 24, 48, and 72 h after inoculation, as compared to non-inoculated plants. Of these 45 proteins, 44 were identified by mass spectrometry analysis using the NCBInr database of Triticum aestivum (26 spots) and closely related species in the Triticum genus (18 spots). These proteins were associated with the defense response, photosynthesis, metabolism, and other cellular processes in wheat. Most of the up-regulated proteins were identified as stress- and defense-related proteins. In particular, the product of a specific powdery mildew resistance gene (Pm3b and its homolog) and some other defense- and pathogenesis-related proteins were overexpressed. The resistance gene product mediates the immune response and coordinates other cellular processes during the resistance response to Bgt.
机译：小麦白粉病是由Blumeria graminis f引起的。 sp。小麦（Bgt）。尽管已经开发了许多对这种疾病具有抗性的小麦品种，但对其抗性机理知之甚少。这项研究的目的是鉴定在被Bgt感染的小麦N0308的抗性反应过程中，蛋白质显示出丰度变化。在二维电泳分析中，与未接种的植物相比，接种后24、48和72 h凝胶上的45个斑点显示出丰度的显着变化。在这45种蛋白质中，使用小麦的NCBInr数据库（26个斑点）和小麦属中密切相关的物种（18个斑点）通过质谱分析鉴定了44种。这些蛋白质与小麦的防御反应，光合作用，代谢和其他细胞过程有关。大多数上调的蛋白质被鉴定为与压力和防御相关的蛋白质。特别是，特定的白粉病抗性基因（Pm3b及其同系物）以及其他一些与防御和发病相关的蛋白质的产物被过表达。抗性基因产物介导免疫反应并在对Bgt的抗性反应中协调其他细胞过程。

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