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首页> 外文期刊>The protein journal >Purification and characterization of antioxidant peptides from leukocyte extract of Crocodylus siamensis.
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Purification and characterization of antioxidant peptides from leukocyte extract of Crocodylus siamensis.

机译：暹罗鳄白细胞提取物中抗氧化肽的纯化和表征。

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Antioxidant peptides were isolated from the leukocyte extract of the Siamese crocodile, Crocodylus siamensis. Crocodile leukocyte was extracted by a combination of methods including freeze-thawing, acetic acid extraction and homogenization. The peptides in the leukocyte extract were purified by anion exchange chromatography and reversed phase-high performance liquid chromatography. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay was used to evaluate the antioxidant activity of the elution peaks at each purification step. As a result, there were two purified peptides exhibiting strong antioxidant activity in reducing free radicals on DPPH molecules. The amino acid sequences of these peptides were determined by LC-MS/MS as TDVLGLPAK (912.5 Da) and DPNAALPAGPR (1,148.6 Da), and their IC?? values were 153.4 and 95.7 μM, respectively. The results of this study therefore indicate that leukocyte extract of C. siamensis contains peptides with antioxidant activity which could be used as a novel antioxidant.
机译：从暹罗鳄（Crocodylus siamensis）的暹罗鳄的白细胞提取物中分离出抗氧化剂肽。通过包括冻融，乙酸提取和均质化在内的多种方法提取鳄鱼白细胞。通过阴离子交换色谱和反相高效液相色谱法纯化白细胞提取物中的肽。 1,1-二苯基-2-吡啶并肼基（DPPH）自由基清除试验用于评估每个纯化步骤洗脱峰的抗氧化活性。结果，有两种纯化的肽在减少DPPH分子上的自由基方面表现出强的抗氧化活性。用LC-MS / MS测定了这些肽的氨基酸序列，分别为TDVLGLPAK（912.5Da）和DPNAALPAGPR（1148.6Da），以及它们的IC 50。值分别为153.4和95.7μM。因此，该研究结果表明，暹罗梭菌的白细胞提取物含有具有抗氧化活性的肽，可以用作新型抗氧化剂。

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