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首页> 外文期刊>The Prostate >Expression of a human cell adhesion molecule, MUC18, in prostate cancer cell lines and tissues.
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Expression of a human cell adhesion molecule, MUC18, in prostate cancer cell lines and tissues.

机译：人细胞粘附分子MUC18在前列腺癌细胞系和组织中的表达。

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摘要

BACKGROUND: Over expression of huMUC18, a cell adhesion molecule in the immunoglobulin gene superfamily, causes a non-metastatic human melanoma cell line to become metastatic in a nude mouse system. To determine if MUC18 expression correlates with the malignant progression of prostate cancer, we investigated differential expression of human MUC18 (huMUC18) in normal prostate epithelial cells, prostate cancer cell lines, and prostatic normal and cancer tissues. METHODS: RT-PCR and Western blot analyses were used to analyze the expression of MUC18 mRNA and protein in four human prostate cancer cell lines, cultured primary normal prostate epithelial cells, normal prostate and malignant prostate tissues. Immunohistochemistry was used to determine the expression of MUC18 antigen in prostatic tissues at different stages of malignancy. RESULTS: Human MUC18 mRNA and protein was expressed in three different prostate cancer cell lines (TSU-PR1, DU145, and PC-3), but not in one prostate cancer cell line (LNCaP.FGC). HuMUC18 protein was also expressed at high levels in extracts prepared from tissue sample sections containing high grade prostatic intraepithelial neoplasia (PIN), but weakly expressed in extracts prepared from either cultured primary normal prostatic epithelial cells or the normal prostate gland. Immunohistochemical analysis showed that huMUC18 was expressed at higher levels in the epithelial cells of high-grade PIN and prostatic carcinomas and in cells of a lymph node metastasis compared to that in normal or benign hyperplastic epithelium (BPH). CONCLUSIONS: We therefore conclude that MUC18 is expressed at higher levels in pre-malignant and malignant prostatic epithelium, including metastasis. We suggest that over-expression of MUC18 may be a new marker of human prostate cancer and also implicates its possible role in development and progression of prostate cancer. Copyright 2001 Wiley-Liss, Inc.
机译：背景：免疫球蛋白基因超家族中的细胞黏附分子huMUC18过度表达，导致非转移性人黑素瘤细胞系在裸鼠系统中转移。为了确定MUC18表达是否与前列腺癌的恶性进展相关，我们研究了人MUC18（huMUC18）在正常前列腺上皮细胞，前列腺癌细胞系以及前列腺正常和癌组织中的差异表达。方法：采用RT-PCR和Western blot方法分析MUC18 mRNA和蛋白在4种人前列腺癌细胞系，培养的原代正常前列腺上皮细胞，正常前列腺和恶性前列腺组织中的表达。免疫组织化学法测定MUC18抗原在恶性肿瘤不同阶段在前列腺组织中的表达。结果：人MUC18 mRNA和蛋白在三种不同的前列腺癌细胞系（TSU-PR1，DU145和PC-3）中表达，但在一种前列腺癌细胞系（LNCaP.FGC）中不表达。从含有高级前列腺上皮内瘤样变（PIN）的组织样品切片制备的提取物中，HuMUC18蛋白也以高水平表达，但在从培养的原代正常前列腺上皮细胞或正常前列腺中提取的提取物中，HuMUC18蛋白的表达却很弱。免疫组织化学分析表明，与正常或良性增生上皮（BPH）相比，huMUC18在高级别PIN和前列腺癌的上皮细胞以及淋巴结转移的细胞中表达更高。结论：因此，我们得出结论，MUC18在恶性前和恶性前列腺上皮（包括转移）中表达较高。我们建议，MUC18的过表达可能是人类前列腺癌的新标志，也暗示了其在前列腺癌的发展和进程中的可能作用。版权所有2001 Wiley-Liss，Inc.

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