...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Journal of Experimental Biology >Pharmacology of ionotropic and metabotropic glutamate receptors on neurons involved in feeding behavior in the pond snail, Helisoma trivolvis
【24h】

Pharmacology of ionotropic and metabotropic glutamate receptors on neurons involved in feeding behavior in the pond snail, Helisoma trivolvis

机译：参与池塘蜗牛tri取食行为的神经元离子型和代谢型谷氨酸受体的药理作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Glutamate is a key regulatory neurotransmitter in the triphasic central pattern generator controlling feeding behavior in the pond snail, Helisoma trivolvis. It excites phase two motor neurons while inhibiting those in phases one and three. However, the receptors that mediate this regulation are only partially characterized. The purpose of these experiments was to further characterize the glutamate receptors on three buccal neurons modulated by glutamate. Intracellular recordings from B5, B19 and B27 neurons were taken during the perfusion of isolated buccal ganglia with agonists that are selective for different vertebrate glutamate receptors. The firing rate of all three neurons was inhibited in a dose-dependent manner by glutamate, including that of B27, a phase 2 motor neuron known to be excited by glutamate in vivo. Quisqualate also reduced the firing rate in all three neurons, and (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), a relatively non-selective metabotropic glutamate receptor (mGluR) agonist, reduced the firing rate in B5 neurons, but not in B19 or B27 neurons. Agonists selective for vertebrate group I, II and III mGluRs did not affect the firing rate in any of the Helisoma buccal neurons tested, suggesting that mGluR agonist binding sites on these neurons do not closely resemble those on any vertebrate mGluR subtypes. An increase in frequency of action potentials was observed in all three cell types in the presence of 100 mu mol l(-1) kainate (KA), suggesting the presence of excitatory (S)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)/KA-like receptors. However, electrotonic coupling between B19 and B27 neurons, and a lack of effect of KA on isolated B19 neurons suggest the excitatory effects of KA on this neuron are indirect. These findings suggest the presence of multiple glutamate receptor subtypes in molluscan neurons that do not always resemble vertebrate receptors pharmacologically.
机译：谷氨酸是三重性中央模式发生器中关键的调节神经递质，它控制着池塘蜗牛Trivolvis的摄食行为。它能激发第二阶段的运动神经元，而抑制第一阶段和第三阶段的神经元。但是，介导该调节的受体仅被部分表征。这些实验的目的是进一步表征由谷氨酸调节的三个颊神经元上的谷氨酸受体。从B5，B19和B27神经元的细胞内记录是在对孤立的颊谷氨酸受体有选择性的激动剂灌注孤立的颊神经节期间进行的。谷氨酸以剂量依赖的方式抑制了所有三个神经元的放电率，包括B27（已知在体内被谷氨酸激发的2期运动神经元B27）的放电率。 Quisqualate还降低了所有三个神经元的放电速率，而（1S，3R）-1-氨基环戊烷-1,3-二羧酸（ACPD）是一种相对非选择性的代谢型谷氨酸受体（mGluR）激动剂，降低了放电速率。 B5神经元，但不在B19或B27神经元中。对脊椎动物I，II和III类mGluRs选择性的激动剂不会影响所测试的任何Helisoma颊神经元的放电速率，这表明这些神经元上的mGluR激动剂结合位点与任何脊椎动物mGluR亚型的结合位点均不太相似。在存在100μmol l（-1）海藻酸酯（KA）的情况下，在所有三种细胞类型中均观察到动作电位的频率增加，表明存在兴奋性（S）-α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）/ KA样受体。但是，B19和B27神经元之间的电渗耦合以及KA对分离的B19神经元缺乏影响表明KA对这种神经元的兴奋作用是间接的。这些发现表明，在软体动物神经元中存在多种谷氨酸受体亚型，它们在药理上并不总是类似于脊椎动物受体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号